PMID- 17166420
OWN - NLM
STAT- MEDLINE
DCOM- 20090507
LR  - 20151119
IS  - 0253-9624 (Print)
IS  - 0253-9624 (Linking)
VI  - 40
IP  - 5
DP  - 2006 Sep
TI  - [Effects of vitamin C on the inhibition of human leucocyte antigen class I 
      (HLA-I) expression of human peripheral blood mononuclear cells induced by 
      deoxynivalenol in vitro].
PG  - 314-8
AB  - OBJECTIVE: To explore the putative effects of Vitamin C (Vit C) on inhibition of 
      human leucocyte antigen class I (HLA-I) expression of human peripheral blood 
      mononuclear cells (HPBMCs) induced by deoxynivalenol (DON) in vitro. METHODS: The 
      effects of Vit C on the changes of HLA-I expression of HPBMCs induced by DON in 
      vitro were evaluated with cell culture, flow cytometry (FCM), Western blotting 
      and immunocytochemical methods. RESULTS: FCM analysis showed that HLA-I 
      expression of HPBMCs in DON treated cells was significantly lower than that in 
      controls (FI 0.88 +/- 0.02 vs 1.00 +/- 0.03, P < 0.05). As compared with DON 
      group, the HLA-I expressions of HPBMCs in the two Vit C (25 micromol/L and 100 
      micromol/L) pretreatment groups were all significantly increased (1.15 +/- 0.06 
      and 1.10 +/- 0.02 vs 0.88 +/- 0.02, P < 0.05). Exposure to different dosage of 
      Vit C alone could dramatically increase the expression of HLA-I of HPBMCs in 
      vitro as compared with that in the normal control (FI for 25 micromol/L and 100 
      micromol/L Vit C treatment group was 1.28 +/- 0.03 and 1.25 +/- 0.05 
      respectively, P < 0.05). Immunocytochemical results showed that the percentages 
      of HLA-I positive expression of HPBMCs in Vit C pretreatment groups at different 
      dosages were significantly higher than those in DON group (70.10 +/- 6.90)%, 
      (64.50 +/- 5.50)% vs (42.20 +/- 4.30)%, P < 0.05. Western blotting confirmed the 
      results of FCM and immunocytochemistry. CONCLUSIONS: Vitamin C pretreatment at 
      different dosages could reverse at some extent the inhibitive effects of DON on 
      HLA-I expression of HPBMCs.
FAU - Zhou, Bing-juan
AU  - Zhou BJ
AD  - Laboratory of Pathology, Hebei Medcial University, Shijiazhuang 050017, China.
FAU - Li, Yue-hong
AU  - Li YH
FAU - Zhang, Xiang-hong
AU  - Zhang XH
FAU - Xing, Ling-xiao
AU  - Xing LX
FAU - Yan, Xia
AU  - Yan X
FAU - Wang, Jun-ling
AU  - Wang JL
FAU - Liu, Jing
AU  - Liu J
FAU - Xing, Xin
AU  - Xing X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yu Fang Yi Xue Za Zhi
JT  - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]
JID - 7904962
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Trichothecenes)
RN  - JT37HYP23V (deoxynivalenol)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Ascorbic Acid/*pharmacology
MH  - Cells, Cultured
MH  - Flow Cytometry
MH  - Histocompatibility Antigens Class I/*metabolism
MH  - Humans
MH  - Monocytes/*drug effects/metabolism
MH  - Trichothecenes/pharmacology
EDAT- 2006/12/15 09:00
MHDA- 2009/05/08 09:00
CRDT- 2006/12/15 09:00
PHST- 2006/12/15 09:00 [pubmed]
PHST- 2009/05/08 09:00 [medline]
PHST- 2006/12/15 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Yu Fang Yi Xue Za Zhi. 2006 Sep;40(5):314-8.