PMID- 1716700
OWN - NLM
STAT- MEDLINE
DCOM- 19911023
LR  - 20201226
IS  - 0022-5347 (Print)
IS  - 0022-5347 (Linking)
VI  - 146
IP  - 4
DP  - 1991 Oct
TI  - Expression of the insulin like growth factor-binding protein 3 (IGFBP-3) gene is 
      increased in human renal carcinomas.
PG  - 1160-3
AB  - After we had established that the IGFBP-3 gene is expressed in normal human 
      kidney we examined renal adenocarcinoma tissue for alterations of the expression 
      of this gene. For this purpose we prepared poly(A)+ RNA from normal kidney tissue 
      and adjacent renal adenocarcinoma of 18 adult patients and compared the levels of 
      IGFBP-3 mRNA by Northern analysis in both samples. The mean content by 
      densitometry was markedly increased in the carcinoma tissues; in 17 of 18 
      patients the carcinoma contained significantly more IGFBP-3 mRNA than the normal 
      kidney sample. The highest mRNA levels were found in patients with N2 and N3 
      lymph node extensions. Comparative Southern analysis of paired samples of four of 
      these patients did not reveal amplification of the gene as the cause of these 
      increased mRNA levels. In one patient, however, we identified a restriction 
      fragment length polymorphism (RFLP) present in normal and malignant cellular DNA. 
      This suggests a participation of the IGFBP-3 gene in the development of human 
      renal cell cancer.
FAU - Hintz, R L
AU  - Hintz RL
AD  - Department of Medicine III, University of Munich Medical School Grosshadern, 
      Germany.
FAU - Bock, S
AU  - Bock S
FAU - Thorsson, A V
AU  - Thorsson AV
FAU - Bovens, J
AU  - Bovens J
FAU - Powell, D R
AU  - Powell DR
FAU - Jakse, G
AU  - Jakse G
FAU - Petrides, P E
AU  - Petrides PE
LA  - eng
GR  - DK24085/DK/NIDDK NIH HHS/United States
GR  - DK38773/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Urol
JT  - The Journal of urology
JID - 0376374
RN  - 0 (Carrier Proteins)
RN  - 0 (Insulin-Like Growth Factor Binding Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Aged
MH  - Blotting, Northern
MH  - Carcinoma, Renal Cell/*genetics
MH  - Carrier Proteins/*genetics
MH  - Densitometry
MH  - Female
MH  - Humans
MH  - Insulin-Like Growth Factor Binding Proteins
MH  - Kidney Neoplasms/*genetics
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/analysis
EDAT- 1991/10/01 00:00
MHDA- 1991/10/01 00:01
CRDT- 1991/10/01 00:00
PHST- 1991/10/01 00:00 [pubmed]
PHST- 1991/10/01 00:01 [medline]
PHST- 1991/10/01 00:00 [entrez]
AID - S0022-5347(17)38031-X [pii]
AID - 10.1016/s0022-5347(17)38031-x [doi]
PST - ppublish
SO  - J Urol. 1991 Oct;146(4):1160-3. doi: 10.1016/s0022-5347(17)38031-x.