PMID- 17169510
OWN - NLM
STAT- MEDLINE
DCOM- 20070531
LR  - 20220318
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 120
IP  - 3-4
DP  - 2007 Mar 10
TI  - Aujeszky's disease virus infection patterns in European wild boar.
PG  - 241-50
AB  - Evidence of exposure (i.e. seroprevalence) to Aujeszky's disease virus (ADV) is 
      high among wild boars from south-central Spain. This research aims to determine 
      the presence of ADV by molecular detection, and to describe the patterns of ADV 
      infection in wild boars. Tonsils (TN) and trigeminal ganglia (TG) for ADV 
      molecular detection, and sera were collected from wild boars (n=192) in 39 
      hunting estates from south-central Spain (2004/2005). A nested polymerase chain 
      reaction (PCR) for a fragment of the ADV surface glycoprotein B was performed on 
      collected tissues. Individual status of presence of viral DNA was tested against 
      explanatory variables by means of a Generalized Linear Mixed Model (GLIMMIX) 
      analysis. Viral detection prevalence was 30.6+/-6.7%. Although there was an 
      increasing pattern with age and females presented higher prevalences, no 
      statistically significant influence of sex and age was found for viral presence. 
      Molecular testing in TN and TG allowed classifying infection status into (i) ADV 
      negative (in both TN and TG), (ii) only positive in TN, (iii) only positive in TG 
      and (iv) positive in both TN and TG. ADV DNA was statistically more frequently 
      evidenced in TN in females than in males. With the exception of one individual, 
      all wild boars with presence of ADV DNA in TN and TG or only in TG reacted 
      positive in the ELISA. In contrast, animals with only ADV DNA in TN serorreacted 
      positively and negatively. Interestingly, 45% of the PCR positive wild boars 
      (n=59) were seronegative in the serological test, all of them with viral DNA only 
      in TN. Our results provide evidence for latency of ADV in wild boars and stress 
      the fact that antibody detection based tests may fail to detect a proportion of 
      recently infected animals. This is of great concern since current management 
      schemes in our study promote animal translocation for hunting purposes, with the 
      associated risk of under-detecting ADV infected individuals when using serology 
      to screen for ADV infection.
FAU - Ruiz-Fons, Francisco
AU  - Ruiz-Fons F
AD  - Instituto de Investigacion en Recursos Cinegeticos IREC (CSIC-UCLM-JCCM), Ronda 
      de Toledo s/n, 13071, Ciudad Real, Spain. jfrfons@irec.uclm.es
FAU - Vidal, Dolo
AU  - Vidal D
FAU - Hofle, Ursula
AU  - Hofle U
FAU - Vicente, Joaquin
AU  - Vicente J
FAU - Gortazar, Christian
AU  - Gortazar C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061212
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - DNA Primers/chemistry
MH  - DNA, Viral/analysis
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Female
MH  - *Herpesvirus 1, Suid/genetics/immunology/isolation & purification
MH  - Male
MH  - Palatine Tonsil/virology
MH  - Polymerase Chain Reaction/veterinary
MH  - Prevalence
MH  - Pseudorabies/*epidemiology
MH  - Spain/epidemiology
MH  - *Sus scrofa
MH  - Trigeminal Ganglion/virology
EDAT- 2006/12/16 09:00
MHDA- 2007/06/01 09:00
CRDT- 2006/12/16 09:00
PHST- 2006/08/08 00:00 [received]
PHST- 2006/10/31 00:00 [revised]
PHST- 2006/11/02 00:00 [accepted]
PHST- 2006/12/16 09:00 [pubmed]
PHST- 2007/06/01 09:00 [medline]
PHST- 2006/12/16 09:00 [entrez]
AID - S0378-1135(06)00442-1 [pii]
AID - 10.1016/j.vetmic.2006.11.003 [doi]
PST - ppublish
SO  - Vet Microbiol. 2007 Mar 10;120(3-4):241-50. doi: 10.1016/j.vetmic.2006.11.003. 
      Epub 2006 Dec 12.