PMID- 1716971
OWN - NLM
STAT- MEDLINE
DCOM- 19911030
LR  - 20011102
IS  - 0921-3775 (Print)
IS  - 0921-3775 (Linking)
VI  - 3
IP  - 2
DP  - 1991
TI  - Immunoassay using the depolarized and forward scattered light intensity 
      fluctuations from latex spheres.
PG  - 113-22
AB  - A new optical method is presented here for detecting immunoreaction by means of 
      the forward and depolarized light scattering by coated carrier particles. By this 
      approach, a short-time measurement of antigen-antibody reactions was concisely 
      achieved. The method covered in this article is based on using double-scattered 
      light. While light that is single-scattered by microspheres is polarized parallel 
      to the incident light polarization, double-scattered light contains depolarized 
      components. The normalized fractional variance in the single-scattered field is 
      inversely proportional to the particle concentration, whereas that in the 
      double-scattered field is inversely proportional to the square of the particle 
      concentration. Due to this difference, the decrease of the particle concentration 
      during the agglutination reaction is more sensitively detected through the 
      measurement of the fractional variance in the double-scattered field. In 
      addition, undesirable light scattered from non-aggregated microspheres is reduced 
      by using the method summarized here. This study is based on the assumption that 
      the particle concentration of the double-scattered field is 4.5 x 10(11) 
      particles/cm3. The latex spheres coated with antibody molecules aggregated after 
      adding the antigen and, as a result, the fractional variance rapidly increased 
      before leveling off after 5-10 min. The antigens measured were alpha-fetoprotein 
      (AFP) and immunoglobulin E (IgE).
FAU - Namba, A
AU  - Namba A
AD  - Olympus Optical Co. Ltd, Tokyo, Japan.
FAU - Musha, T
AU  - Musha T
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Front Med Biol Eng
JT  - Frontiers of medical and biological engineering : the international journal of 
      the Japan Society of Medical Electronics and Biological Engineering
JID - 9011464
RN  - 0 (Latex)
RN  - 0 (alpha-Fetoproteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antigen-Antibody Reactions
MH  - Equipment Design
MH  - Immunoassay/*methods
MH  - Immunoglobulin E/analysis
MH  - Lasers
MH  - Latex
MH  - *Light
MH  - *Microspheres
MH  - alpha-Fetoproteins/analysis
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
PST - ppublish
SO  - Front Med Biol Eng. 1991;3(2):113-22.