PMID- 17169924
OWN - NLM
STAT- MEDLINE
DCOM- 20070510
LR  - 20151119
IS  - 0032-0781 (Print)
IS  - 0032-0781 (Linking)
VI  - 48
IP  - 2
DP  - 2007 Feb
TI  - Characterization of transgenic Arabidopsis plants overexpressing high mobility 
      group B proteins under high salinity, drought or cold stress.
PG  - 221-31
AB  - High mobility group B (HMGB) proteins found in the nuclei of higher eukaryotes 
      play roles in various cellular processes such as replication, transcription and 
      nucleosome assembly. The Arabidopsis thaliana genome contains eight genes 
      encoding HMGB proteins, the functions of which remain largely unknown in the 
      transcriptional regulation of plant stress responses. To understand better the 
      functions of HMGB proteins in the responses of plants to environmental stimuli, 
      we examined the effect of various abiotic stresses on germination and growth of 
      transgenic Arabidopsis plants that overexpress a single isoform of HMGB. The 
      expression of HMGB2, HMGB3 and HMGB4 was up-regulated by cold stress, whereas the 
      expression of HMGB2 and HMGB3 was markedly down-regulated by drought or salt 
      stress. Under salt or drought stress, the transgenic Arabidopsis plants that 
      overexpress HMGB2 displayed retarded germination and subsequent growth compared 
      with wild-type plants. Overexpression of HMGB4 had no impact on seed germination 
      and seedling growth of the plants under the stress conditions tested. In contrast 
      to no significant stress-related phenotypes of HMGB5-overexpressing plants, 
      loss-of-function mutants of HMGB5 displayed retarded germination and subsequent 
      growth compared with wild-type plants under stress conditions. Although 
      transcript levels of various stress-responsive genes were not modulated by the 
      expression of HMGB2, expression of several germination-responsive genes was 
      modulated by HMGB2 under salt stress. Taken together, these results provide a 
      novel basis for understanding the biological functions of HMGB protein family 
      members that differently affect germination and seedling growth of Arabidopsis 
      plants under various stress conditions.
FAU - Kwak, Kyung Jin
AU  - Kwak KJ
AD  - Department of Plant Biotechnology, Agricultural Plant Stress Research Center and 
      Biotechnology Research Institute, College of Agriculture and Life Sciences, 
      Chonnam National University, Gwangju, 500-757 Korea.
FAU - Kim, Joo Yeol
AU  - Kim JY
FAU - Kim, Yeon Ok
AU  - Kim YO
FAU - Kang, Hunseung
AU  - Kang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061214
PL  - Japan
TA  - Plant Cell Physiol
JT  - Plant & cell physiology
JID - 9430925
RN  - 0 (High Mobility Group Proteins)
RN  - 451W47IQ8X (Sodium Chloride)
SB  - IM
MH  - Arabidopsis/*genetics/physiology
MH  - *Cold Temperature
MH  - *Disasters
MH  - Genes, Plant
MH  - High Mobility Group Proteins/*genetics/metabolism
MH  - Plants, Genetically Modified
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Sodium Chloride
EDAT- 2006/12/16 09:00
MHDA- 2007/05/11 09:00
CRDT- 2006/12/16 09:00
PHST- 2006/12/16 09:00 [pubmed]
PHST- 2007/05/11 09:00 [medline]
PHST- 2006/12/16 09:00 [entrez]
AID - pcl057 [pii]
AID - 10.1093/pcp/pcl057 [doi]
PST - ppublish
SO  - Plant Cell Physiol. 2007 Feb;48(2):221-31. doi: 10.1093/pcp/pcl057. Epub 2006 Dec 
      14.