PMID- 1717009
OWN - NLM
STAT- MEDLINE
DCOM- 19911029
LR  - 20190918
IS  - 0891-6934 (Print)
IS  - 0891-6934 (Linking)
VI  - 8
IP  - 1
DP  - 1990
TI  - Demonstration of IgE antibodies to nucleic acid antigens in patients with SLE.
PG  - 59-64
AB  - Using a combination of avidin-biotin microELISA and solid phase radioimmunoassay, 
      we examined sera from 23 patients with systemic lupus erythematosus (SLE), two 
      patients with established sensitivity to ingested shrimp, and 15 healthy normal 
      subjects. In addition to IgG antibodies, varying amounts of IgE antibodies 
      specific for native DNA (nDNA), denatured or single-stranded DNA (dnDNA), RNA, 
      and tRNA were demonstrable in the sera of SLE patients, but not in the sera of 
      normal subjects. A comparison of the specificity of nucleic acid-specific IgE 
      antibodies present in the sera of shrimp-sensitive patients with those present in 
      the sera of seven SLE patients revealed that the IgE antibodies in the sera of 
      shrimp-sensitive patients specifically recognized shrimp tRNA but not yeast tRNA, 
      calf thymus RNA, or calf thymus DNA, while those present in the sera of patients 
      with SLE recognized all these nucleic acid antigens. The IgE antibodies directed 
      against nDNA, dnDNA, RNA, and tRNA may mediate mast cell and basophil 
      degranulation and thus contribute both to immediate-type hypersensitivity 
      phenomena including hives seen in patients with SLE and to the localization of 
      IgE-nucleic acid complexes in target tissues.
FAU - Nagpal, S
AU  - Nagpal S
AD  - Department of Biochemistry, Indian Institute of Science, Bangalore, India.
FAU - Sriramarao, P
AU  - Sriramarao P
FAU - Krishnaswamy, P R
AU  - Krishnaswamy PR
FAU - Metcalfe, D D
AU  - Metcalfe DD
FAU - Rao, P V
AU  - Rao PV
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Autoimmunity
JT  - Autoimmunity
JID - 8900070
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (DNA, Single-Stranded)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - 9014-25-9 (RNA, Transfer)
SB  - IM
MH  - Antibodies, Antinuclear/*analysis
MH  - DNA/immunology
MH  - DNA, Single-Stranded/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/analysis
MH  - Lupus Erythematosus, Systemic/*immunology
MH  - Male
MH  - RNA/immunology
MH  - RNA, Transfer/immunology
MH  - Radioimmunoassay
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.3109/08916939008998433 [doi]
PST - ppublish
SO  - Autoimmunity. 1990;8(1):59-64. doi: 10.3109/08916939008998433.