PMID- 17170124
OWN - NLM
STAT- MEDLINE
DCOM- 20070523
LR  - 20220129
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 109
IP  - 8
DP  - 2007 Apr 15
TI  - Five members of the CEBP transcription factor family are targeted by recurrent 
      IGH translocations in B-cell precursor acute lymphoblastic leukemia (BCP-ALL).
PG  - 3451-61
AB  - CCAAT enhancer-binding protein (CEBP) transcription factors play pivotal roles in 
      proliferation and differentiation, including suppression of myeloid 
      leukemogenesis. Mutations of CEBPA are found in a subset of acute myeloid 
      leukemia (AML) and in some cases of familial AML. Here, using cytogenetics, 
      fluorescence in situ hybridization (FISH), and molecular cloning, we show that 5 
      CEBP gene family members are targeted by recurrent IGH chromosomal translocations 
      in BCP-ALL. Ten patients with t(8;14)(q11;q32) involved CEBPD on chromosome 8, 
      and 9 patients with t(14;19)(q32;q13) involved CEBPA, while a further patient 
      involved CEBPG, located 71 kb telomeric of CEBPA in chromosome band 19q13; 4 
      patients with inv(14)(q11q32)/t(14;14)(q11;q32) involved CEBPE and 3 patients 
      with t(14;20)(q32;q13) involved CEBPB. In 16 patients the translocation 
      breakpoints were cloned using long-distance inverse-polymerase chain reaction 
      (LDI-PCR). With the exception of CEBPD breakpoints, which were scattered within a 
      43-kb region centromeric of CEBPD, translocation breakpoints were clustered 
      immediately 5' or 3' of the involved CEBP gene. Except in 1 patient with 
      t(14;14)(q11;q32), the involved CEBP genes retained germ-line sequences. 
      Quantitative reverse transcription (RT)-PCR showed overexpression of the 
      translocated CEBP gene. Our findings implicate the CEBP gene family as novel 
      oncogenes in BCP-ALL, and suggest opposing functions of CEBP dysregulation in 
      myeloid and lymphoid leukemogenesis.
FAU - Akasaka, Takashi
AU  - Akasaka T
AD  - Toxicology Unit, Medical Research Council, University of Leicester, Lancaster 
      Road, Leicester, UK.
FAU - Balasas, Theodore
AU  - Balasas T
FAU - Russell, Lisa J
AU  - Russell LJ
FAU - Sugimoto, Kei-ji
AU  - Sugimoto KJ
FAU - Majid, Aneela
AU  - Majid A
FAU - Walewska, Renata
AU  - Walewska R
FAU - Karran, E Loraine
AU  - Karran EL
FAU - Brown, David G
AU  - Brown DG
FAU - Cain, Kelvin
AU  - Cain K
FAU - Harder, Lana
AU  - Harder L
FAU - Gesk, Stefan
AU  - Gesk S
FAU - Martin-Subero, Jose Ignacio
AU  - Martin-Subero JI
FAU - Atherton, Mark G
AU  - Atherton MG
FAU - Bruggemann, Monika
AU  - Bruggemann M
FAU - Calasanz, Maria Jose
AU  - Calasanz MJ
FAU - Davies, Teresa
AU  - Davies T
FAU - Haas, Oskar A
AU  - Haas OA
FAU - Hagemeijer, Anne
AU  - Hagemeijer A
FAU - Kempski, Helena
AU  - Kempski H
FAU - Lessard, Michel
AU  - Lessard M
FAU - Lillington, Debra M
AU  - Lillington DM
FAU - Moore, Sarah
AU  - Moore S
FAU - Nguyen-Khac, Florence
AU  - Nguyen-Khac F
FAU - Radford-Weiss, Isabelle
AU  - Radford-Weiss I
FAU - Schoch, Claudia
AU  - Schoch C
FAU - Struski, Stephanie
AU  - Struski S
FAU - Talley, Polly
AU  - Talley P
FAU - Welham, Melanie J
AU  - Welham MJ
FAU - Worley, Helen
AU  - Worley H
FAU - Strefford, Jon C
AU  - Strefford JC
FAU - Harrison, Christine J
AU  - Harrison CJ
FAU - Siebert, Reiner
AU  - Siebert R
FAU - Dyer, Martin J S
AU  - Dyer MJ
LA  - eng
GR  - MC_U132670597/MRC_/Medical Research Council/United Kingdom
PT  - Clinical Trial
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20061214
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (Immunoglobulin Heavy Chains)
SB  - IM
MH  - Burkitt Lymphoma/*genetics
MH  - CCAAT-Enhancer-Binding Proteins/*genetics
MH  - Centromere/genetics
MH  - Chromosomes, Human/*genetics
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Multigene Family/*genetics
MH  - Oncogenes/*genetics
MH  - Polymerase Chain Reaction
MH  - Telomere/genetics
MH  - *Translocation, Genetic
EDAT- 2006/12/16 09:00
MHDA- 2007/05/24 09:00
CRDT- 2006/12/16 09:00
PHST- 2006/12/16 09:00 [pubmed]
PHST- 2007/05/24 09:00 [medline]
PHST- 2006/12/16 09:00 [entrez]
AID - S0006-4971(20)41722-7 [pii]
AID - 10.1182/blood-2006-08-041012 [doi]
PST - ppublish
SO  - Blood. 2007 Apr 15;109(8):3451-61. doi: 10.1182/blood-2006-08-041012. Epub 2006 
      Dec 14.