PMID- 17170125
OWN - NLM
STAT- MEDLINE
DCOM- 20070523
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 109
IP  - 8
DP  - 2007 Apr 15
TI  - TLR agonists induce regulatory dendritic cells to recruit Th1 cells via 
      preferential IP-10 secretion and inhibit Th1 proliferation.
PG  - 3308-15
AB  - Dendritic cells (DCs) and chemokines are important mediators linking innate and 
      adaptive immunity on activation by Toll-like receptor (TLR) agonists. We 
      previously identified a kind of regulatory DC subset (diffDCs) that 
      differentiated from mature DCs under splenic stroma and that inhibited T-cell 
      proliferation. The responsiveness of such regulatory DCs to TLR agonists and 
      their pattern of chemokine production remain to be determined. Here, we report 
      that the regulatory DCs secrete a higher level of CXCR3 chemokine 
      IFN-gamma-induced protein-10 (IP-10) than immature DCs (imDCs), and more IP-10 is 
      produced after stimulation with TLR-2, -4, -3, and -9 ligands. Blockade of 
      IFN-alpha/beta inhibits IP-10 production by TLR agonist-activated regulatory DCs. 
      We show that the increased IRF-3 and IFN-beta-induced STAT1 activation are 
      responsible for the autocrine IFN-beta-dependent preferential production of IP-10 
      by regulatory DCs. In addition, stimulation with recombinant mouse IFN-alpha/beta 
      induces more IP-10 production in regulatory DCs than that in imDCs. Moreover, the 
      regulatory DCs selectively recruit more Th1 cells through IP-10 and inhibit Th1 
      proliferation. Our results demonstrate a new manner for regulatory DCs to 
      down-regulate T-cell response by preferential IP-10 production and inhibition of 
      recruited Th1 cell proliferation.
FAU - Qian, Cheng
AU  - Qian C
AD  - Institute of Immunology and National Key Laboratory of Medical Immunology, Second 
      Military Medical University, 800 Xiangyin Road, Shanghai 200433, People's 
      Republic of China.
FAU - An, Huazhang
AU  - An H
FAU - Yu, Yizhi
AU  - Yu Y
FAU - Liu, Shuxun
AU  - Liu S
FAU - Cao, Xuetao
AU  - Cao X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061214
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Interferon Regulatory Factor-3)
RN  - 0 (Irf3 protein, mouse)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Toll-Like Receptors)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Chemokine CXCL10
MH  - Chemokines, CXC/immunology/*metabolism
MH  - Dendritic Cells/immunology/*metabolism
MH  - Interferon Regulatory Factor-3/immunology
MH  - Interferons/immunology/pharmacology
MH  - Lipopolysaccharides/*pharmacology
MH  - Mice
MH  - Spleen/immunology
MH  - Stromal Cells/immunology
MH  - Th1 Cells/*immunology/metabolism
MH  - Toll-Like Receptors/*agonists/immunology
EDAT- 2006/12/16 09:00
MHDA- 2007/05/24 09:00
CRDT- 2006/12/16 09:00
PHST- 2006/12/16 09:00 [pubmed]
PHST- 2007/05/24 09:00 [medline]
PHST- 2006/12/16 09:00 [entrez]
AID - S0006-4971(20)41705-7 [pii]
AID - 10.1182/blood-2006-08-040337 [doi]
PST - ppublish
SO  - Blood. 2007 Apr 15;109(8):3308-15. doi: 10.1182/blood-2006-08-040337. Epub 2006 
      Dec 14.