PMID- 17171500
OWN - NLM
STAT- MEDLINE
DCOM- 20070403
LR  - 20161124
IS  - 0171-967X (Print)
IS  - 0171-967X (Linking)
VI  - 79
IP  - 6
DP  - 2006 Dec
TI  - Calcification inhibitors and Wnt signaling proteins are implicated in bovine 
      artery smooth muscle cell calcification in the presence of phosphate and vitamin 
      D sterols.
PG  - 431-42
AB  - Administration of active vitamin D sterols to treat secondary hyperparathyroidism 
      in patients with chronic kidney disease receiving dialysis has been associated 
      with elevated serum calcium and phosphorus levels, which may lead to increased 
      risk of vascular calcification. However, calcimimetics, by binding to the 
      parathyroid gland calcium-sensing receptors, reduce serum parathyroid hormone, 
      calcium, phosphorus, and the calcium-phosphorus product. Using cultured bovine 
      aorta vascular smooth muscle cells (BASMCs), an in vitro model of vascular 
      calcification, we compared calcification levels and gene expression profiles 
      after exposure to the phosphate source ss-glycerolphosphate (BGP), the active 
      vitamin D sterols calcitriol and paricalcitol, the calcimimetic R-568, or BGP 
      with the active vitamin D sterols or R-568. Cells exposed to BGP (10 mM) alone or 
      with calcitriol or paricalcitol showed dose-dependent BASMC calcification. No 
      change in calcification was observed in cultures exposed to BGP with R-568, 
      consistent with the observed lack of calcium-sensing receptor expression. 
      Microarray analysis using total cellular RNA from cultures exposed to vehicle or 
      BGP in the absence and presence of 10(-8) M calcitriol or paricalcitol for 7 days 
      showed that cells exposed to BGP with calcitriol or BGP with paricalcitol had 
      virtually identical gene expression profiles, which differed from those of cells 
      treated with BGP or vehicle alone. Several osteoblast- and chondrocyte-associated 
      genes were modulated by BGP and vitamin D exposure. In this study, exposure of 
      BASMCs to phosphate and active vitamin D sterols induced calcification and 
      changes in expression of genes associated with mineralized tissue.
FAU - Shalhoub, V
AU  - Shalhoub V
AD  - Department of Metabolic Disorders, Amgen, Inc., One Amgen Center Drive, Thousand 
      Oaks, CA 91320, USA. shalhoub@amgen.com
FAU - Shatzen, E
AU  - Shatzen E
FAU - Henley, C
AU  - Henley C
FAU - Boedigheimer, M
AU  - Boedigheimer M
FAU - McNinch, J
AU  - McNinch J
FAU - Manoukian, R
AU  - Manoukian R
FAU - Damore, M
AU  - Damore M
FAU - Fitzpatrick, D
AU  - Fitzpatrick D
FAU - Haas, K
AU  - Haas K
FAU - Twomey, B
AU  - Twomey B
FAU - Kiaei, P
AU  - Kiaei P
FAU - Ward, S
AU  - Ward S
FAU - Lacey, D L
AU  - Lacey DL
FAU - Martin, D
AU  - Martin D
LA  - eng
PT  - Journal Article
DEP - 20061208
PL  - United States
TA  - Calcif Tissue Int
JT  - Calcified tissue international
JID - 7905481
RN  - 0 (Aniline Compounds)
RN  - 0 (Drug Combinations)
RN  - 0 (Ergocalciferols)
RN  - 0 (Glycerophosphates)
RN  - 0 (N-(2-chlorophenylpropyl)-1-(3-methoxyphenyl)ethylamine)
RN  - 0 (Phenethylamines)
RN  - 0 (Propylamines)
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (Receptors, Calcium-Sensing)
RN  - 0 (Wnt Proteins)
RN  - 27YLU75U4W (Phosphorus)
RN  - 6702D36OG5 (paricalcitol)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - FXC9231JVH (Calcitriol)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Aniline Compounds/*pharmacology
MH  - Animals
MH  - Aorta/drug effects/metabolism/pathology
MH  - Calcinosis/chemically induced/metabolism/*prevention & control
MH  - Calcitriol/*pharmacology
MH  - Calcium/agonists/metabolism/pharmacology
MH  - Cattle
MH  - Cells, Cultured
MH  - Drug Combinations
MH  - Ergocalciferols/*pharmacology
MH  - Gene Expression/drug effects
MH  - Glycerophosphates/*pharmacology
MH  - Muscle, Smooth, Vascular/*drug effects/metabolism/pathology
MH  - Oligonucleotide Array Sequence Analysis
MH  - Phenethylamines
MH  - Phosphorus/metabolism/pharmacology
MH  - Propylamines
MH  - Receptors, Calcitriol/genetics/metabolism
MH  - Receptors, Calcium-Sensing/drug effects/genetics/metabolism
MH  - Signal Transduction
MH  - Wnt Proteins/*physiology
EDAT- 2006/12/16 09:00
MHDA- 2007/04/04 09:00
CRDT- 2006/12/16 09:00
PHST- 2006/05/02 00:00 [received]
PHST- 2006/08/12 00:00 [accepted]
PHST- 2006/12/16 09:00 [pubmed]
PHST- 2007/04/04 09:00 [medline]
PHST- 2006/12/16 09:00 [entrez]
AID - 10.1007/s00223-006-0126-z [doi]
PST - ppublish
SO  - Calcif Tissue Int. 2006 Dec;79(6):431-42. doi: 10.1007/s00223-006-0126-z. Epub 
      2006 Dec 8.