PMID- 17172132
OWN - NLM
STAT- MEDLINE
DCOM- 20070111
LR  - 20191210
IS  - 0005-9366 (Print)
IS  - 0005-9366 (Linking)
VI  - 119
IP  - 11-12
DP  - 2006 Nov-Dec
TI  - Analysis of fluoroquinolone residues in edible chicken tissues using 
      supercritical fluid extraction.
PG  - 456-60
AB  - We evaluated a procedure that uses high-performance liquid chromatography to 
      determine the levels of four fluoroquinolones (ciprofloxacin, enrofloxacin, 
      norfloxacin, and ofloxacin) remaining in chicken breast muscle, liver, and 
      kidneys after the analytes are extracted using supercritical fluid extraction 
      (SFE). These antimicrobial fluoroquinolones were extracted by SFE using 
      supercritical CO2 containing 30% (v/v) methanol. The recovery, limit of 
      detection, accuracy, and precision of this method were evaluated on the basis of 
      fortified matrices at concentrations of 25 to 250 microg/kg. The method is 
      validated and shown to be linear in the range of 2.5-50 microg/kg. Spike 
      recoveries for muscle, liver and kidney samples prepared at 4 spiking levels were 
      ranged from 56.6-104.3%, 51.2-98.4% and 62.2-97.8%, respectively. The coefficient 
      of variation (CV) for recovery as a measure of relative variability was between 
      3% and 13%, and the relative standard deviation (RSD) was < 20%. The limits of 
      quantitation (LOQ) were 10 microg/kg for enrofloxacin, 12.5 microg/kg each for 
      ciprofloxacin and norfloxacin and 25 microg/kg for ofloxacin; these values were 
      lower than the maximum residue levels (MRLs) authorized by the European 
      Community. The 4 compounds were evaluated simultaneously, and the method was 
      shown to be applicable for analyzing their residues in edible chicken tissues.
FAU - Choi, Jeong Heui
AU  - Choi JH
AD  - Natural Products Chemistry Laboratory, Institute of Agricultural Science and 
      Technology, Chonnam National University, Yong-Bong Dong, Buk-Ku, Gwangju, 
      Republic of Korea.
FAU - Abd El-Aty, A M
AU  - Abd El-Aty AM
FAU - Shen, Jing Yu
AU  - Shen JY
FAU - Kim, Mi Ra
AU  - Kim MR
FAU - Shim, Jae-Han
AU  - Shim JH
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Berl Munch Tierarztl Wochenschr
JT  - Berliner und Munchener tierarztliche Wochenschrift
JID - 0003163
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Fluoroquinolones)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/analysis
MH  - Chickens/metabolism
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Consumer Product Safety
MH  - Drug Residues/*analysis
MH  - Fluoroquinolones/*analysis
MH  - Food Contamination/*analysis
MH  - Humans
MH  - Meat/*analysis
MH  - Organ Specificity
MH  - Sensitivity and Specificity
EDAT- 2006/12/19 09:00
MHDA- 2007/01/12 09:00
CRDT- 2006/12/19 09:00
PHST- 2006/12/19 09:00 [pubmed]
PHST- 2007/01/12 09:00 [medline]
PHST- 2006/12/19 09:00 [entrez]
PST - ppublish
SO  - Berl Munch Tierarztl Wochenschr. 2006 Nov-Dec;119(11-12):456-60.