PMID- 17173248
OWN - NLM
STAT- MEDLINE
DCOM- 20070405
LR  - 20220408
IS  - 1121-8428 (Print)
IS  - 1121-8428 (Linking)
VI  - 19
IP  - 6
DP  - 2006 Nov-Dec
TI  - Glomerular expression of CTGF, TGF-beta 1 and type IV collagen in diabetic 
      nephropathy.
PG  - 751-7
AB  - BACKGROUND: In development of progressive extracellular matrix accumulation, 
      connective tissue growth factor (CTGF) may act as a downstream mediator of 
      transforming growth factor-beta 1 (TGF-beta 1). However, the association and the 
      correlation of these cytokines and extracellular matrix accumulation in human 
      diabetic nephropathy (DN) is not fully understood. METHODS: To explore the 
      possible involvement of TGF-beta 1 and CTGF in extracellular matrix accumulation 
      in DN, high-resolution in situ hybridization with digoxigenin-labeled antisense 
      oligonucleotides to CTGF, TGF-beta 1 and type IV collagen mRNAs were performed in 
      DN and in histologically normal human kidney (NHK). To quantify expression of 
      each mRNA, the fraction of all nuclear cells that were positively stained in the 
      cytoplasm was determined in at least 10 randomly selected cross-sections of 
      nonsclerotic glomeruli. RESULTS: Both in DN and in NHK, CTGF, TGF-beta 1 and type 
      IV collagen mRNAs were mainly expressed by glomerular mesangial, visceral 
      epithelial and parietal epithelial cells. The percentages of positive glomerular 
      resident cells were significantly higher for each mRNA in DN compared with NHK. 
      Especially, the expression of CTGF mRNA was also notably increased in case of DN 
      with only mild histopathologic lesions. The extent of expression of each mRNA was 
      significantly correlated to that of each other mRNA examined. CONCLUSION: Our 
      study indicated that CTGF and TGF-beta may play an important role in glomerular 
      histopathologic change in DN.
FAU - Umezono, Tomoya
AU  - Umezono T
AD  - Division of Nephrology and Metabolism, Department of Internal Medicine, Tokai 
      University, School of Medicine, Kanagawa, Japan. umechan@is.icc.u-tokai.ac.jp
FAU - Toyoda, Masao
AU  - Toyoda M
FAU - Kato, Mayuko
AU  - Kato M
FAU - Miyauchi, Masaaki
AU  - Miyauchi M
FAU - Kimura, Moritugu
AU  - Kimura M
FAU - Maruyama, Mayumi
AU  - Maruyama M
FAU - Honma, Masashi
AU  - Honma M
FAU - Yagame, Mitsunori
AU  - Yagame M
FAU - Suzuki, Daisuke
AU  - Suzuki D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Randomized Controlled Trial
PL  - Italy
TA  - J Nephrol
JT  - Journal of nephrology
JID - 9012268
RN  - 0 (CCN2 protein, human)
RN  - 0 (Collagen Type IV)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (messenger ribonucleoprotein)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
SB  - IM
MH  - Adult
MH  - Collagen Type IV/*biosynthesis
MH  - Connective Tissue Growth Factor
MH  - Diabetic Nephropathies/*metabolism/pathology
MH  - Female
MH  - Glomerular Mesangium/*metabolism/pathology
MH  - Humans
MH  - Immediate-Early Proteins/*biosynthesis
MH  - In Situ Hybridization/methods
MH  - Intercellular Signaling Peptides and Proteins/*biosynthesis
MH  - Male
MH  - Middle Aged
MH  - Podocytes/*metabolism/pathology
MH  - Ribonucleoproteins/biosynthesis
MH  - Transforming Growth Factor beta1/*biosynthesis
EDAT- 2006/12/19 09:00
MHDA- 2007/04/06 09:00
CRDT- 2006/12/19 09:00
PHST- 2006/12/19 09:00 [pubmed]
PHST- 2007/04/06 09:00 [medline]
PHST- 2006/12/19 09:00 [entrez]
PST - ppublish
SO  - J Nephrol. 2006 Nov-Dec;19(6):751-7.