PMID- 17173374
OWN - NLM
STAT- MEDLINE
DCOM- 20070309
LR  - 20220129
IS  - 0893-228X (Print)
IS  - 0893-228X (Linking)
VI  - 19
IP  - 12
DP  - 2006 Dec
TI  - Biomarkers for isocyanate exposure: Synthesis of isocyanate DNA adducts.
PG  - 1611-8
AB  - Isocyanates are important intermediates in industrial manufacturing. DNA adducts 
      and protein adducts are important tools to biomonitor people exposed to 
      xenobiotics. In the present work, the formation of DNA adducts deriving from 
      4-chlorophenyl isocyanate (4CPI) and 4-methylphenyl isocyanate (4MPI) were 
      explored. The adducts of 4CPI and/or 4MPI with 2'-deoxyadenosine, 
      2'-deoxyguanosine, and 2'-deoxycytidine were synthesized and characterized by NMR 
      and MS. For low level detection, an LC-MS/MS method was developed. The formation 
      of DNA adducts was confirmed in in vitro reactions with DNA.
FAU - Beyerbach, Armin
AU  - Beyerbach A
AD  - Institute of Environmental and Occupational Toxicology, Casella Postale 108, 
      CH-6780 Airolo, Switzerland.
FAU - Farmer, Peter B
AU  - Farmer PB
FAU - Sabbioni, Gabriele
AU  - Sabbioni G
LA  - eng
GR  - G0100873/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Chem Res Toxicol
JT  - Chemical research in toxicology
JID - 8807448
RN  - 0 (Biomarkers)
RN  - 0 (DNA Adducts)
RN  - 0 (Isocyanates)
RN  - 0 (Nucleosides)
SB  - IM
MH  - Biomarkers/chemistry
MH  - Chromatography, High Pressure Liquid
MH  - *DNA Adducts/chemical synthesis/chemistry
MH  - Environmental Exposure/*analysis
MH  - Isocyanates/*chemistry
MH  - Molecular Structure
MH  - Nuclear Magnetic Resonance, Biomolecular
MH  - Nucleosides/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization
EDAT- 2006/12/19 09:00
MHDA- 2007/03/10 09:00
CRDT- 2006/12/19 09:00
PHST- 2006/12/19 09:00 [pubmed]
PHST- 2007/03/10 09:00 [medline]
PHST- 2006/12/19 09:00 [entrez]
AID - 10.1021/tx0600853 [doi]
PST - ppublish
SO  - Chem Res Toxicol. 2006 Dec;19(12):1611-8. doi: 10.1021/tx0600853.