PMID- 17174123
OWN - NLM
STAT- MEDLINE
DCOM- 20070207
LR  - 20061218
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 25
IP  - 6
DP  - 2006 Dec
TI  - Dendritic cells regulate exposure of MHC class II at their plasma membrane by 
      oligoubiquitination.
PG  - 885-94
AB  - Dendritic cells (DCs) initiate adaptive immune responses by activating T cells 
      via cognate interactions between MHC-peptide complexes and T cell receptors. In 
      immature DCs, MHC class II is predominantly stored in late endocytic 
      compartments, where it has a short half-life because of degradation. In contrast, 
      mature DCs recruit MHC class II to the plasma membrane. We here demonstrate that 
      in immature DCs, the beta-chain of MHC class II was oligoubiquitinated after 
      proteolytic processing of the associated invariant chain in endosomes and that 
      this modification was required for efficient endocytosis and sorting into luminal 
      vesicles of multivesicular bodies. Ubiquitination of MHC class II was suppressed 
      in lipopolysaccharide-activated DCs. Mutated MHC class II lacking its 
      ubiquitination site was expressed at the plasma membrane, irrespective of DC 
      maturation. Together, these data provide a molecular basis for the regulation of 
      MHC class II-mediated antigen presentation by DCs.
FAU - van Niel, Guillaume
AU  - van Niel G
AD  - Faculty of Veterinary Medicine, Department of Biochemistry & Cell Biology, 
      Utrecht University, P.O. Box 80.176, NL-3508 TD, Utrecht, The Netherlands.
FAU - Wubbolts, Richard
AU  - Wubbolts R
FAU - Ten Broeke, Toine
AU  - Ten Broeke T
FAU - Buschow, Sonja I
AU  - Buschow SI
FAU - Ossendorp, Ferry A
AU  - Ossendorp FA
FAU - Melief, Cornelis J
AU  - Melief CJ
FAU - Raposo, Graca
AU  - Raposo G
FAU - van Balkom, Bas W
AU  - van Balkom BW
FAU - Stoorvogel, Willem
AU  - Stoorvogel W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Ubiquitin)
SB  - IM
CIN - Immunity. 2006 Dec;25(6):857-9. PMID: 17174926
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cell Membrane/immunology/*metabolism
MH  - Dendritic Cells/cytology/immunology/*metabolism
MH  - Endocytosis/immunology
MH  - Endosomes/metabolism
MH  - Histocompatibility Antigens Class II/immunology/*metabolism
MH  - Immunoprecipitation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Confocal
MH  - Microscopy, Immunoelectron
MH  - Ubiquitin/*metabolism
EDAT- 2006/12/19 09:00
MHDA- 2007/02/08 09:00
CRDT- 2006/12/19 09:00
PHST- 2006/10/12 00:00 [received]
PHST- 2006/11/13 00:00 [accepted]
PHST- 2006/12/19 09:00 [pubmed]
PHST- 2007/02/08 09:00 [medline]
PHST- 2006/12/19 09:00 [entrez]
AID - S1074-7613(06)00516-4 [pii]
AID - 10.1016/j.immuni.2006.11.001 [doi]
PST - ppublish
SO  - Immunity. 2006 Dec;25(6):885-94. doi: 10.1016/j.immuni.2006.11.001.