PMID- 17174746
OWN - NLM
STAT- MEDLINE
DCOM- 20070202
LR  - 20061218
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 67
IP  - 12
DP  - 2006 Dec
TI  - Human bone marrow stromal cells hamper specific interactions of CD4 and CD8 T 
      lymphocytes with antigen-presenting cells.
PG  - 976-85
AB  - Bone marrow stromal cells (BMSCs) may inhibit T-cell functions in vitro and thus 
      have been proposed as immunoregulators to control in vivo graft-versus-host 
      disease (GVHD) in haploidentical hemopoietic stem cell transplants. To better 
      investigate this phenomenon, we used a defined experimental system in which 
      responding T cells are antigen-specific and devoid of alloreactivity against BMSC 
      from a different subject. Thus, we established antigen-specific human CD4 and CD8 
      T-cell lines as the readout system. Antigen-dependent proliferation was reduced 
      with both T-cell subsets cultured on confluent BMSCs, and also on confluent human 
      skin fibroblasts (HSF) inhibited T-cell proliferation with similar efficiency. 
      Morphological observations of the cocultures showed impairment of physical 
      interactions between T-cell and antigen-presenting cells in the presence of BMSC, 
      with lack of formation of antigen-dependent clusters of T cells and 
      antigen-presenting cells (APCs). In contrast, no effects were seen with 
      BMSC-conditioned medium. Since suppression was seen only with confluent 
      mesenchymal cells, this phenomenon may not be relevant in vivo, where BMSCs are 
      at low frequency. In addition, if the reported suppressive effect of BMSCs on 
      GVHD in vivo is confirmed, a different in vitro system should be envisaged to 
      better understand and exploit the underlying mechanism.
FAU - Li Pira, Giuseppina
AU  - Li Pira G
AD  - Advanced Biotechnology Center, Genoa, Italy. lipira@email.it
FAU - Ivaldi, Federico
AU  - Ivaldi F
FAU - Bottone, Laura
AU  - Bottone L
FAU - Quarto, Rodolfo
AU  - Quarto R
FAU - Manca, Fabrizio
AU  - Manca F
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061018
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigen-Presenting Cells/*immunology
MH  - Bone Marrow Cells/*immunology
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cell Communication/*immunology
MH  - Cell Line
MH  - Child
MH  - Child, Preschool
MH  - Coculture Techniques
MH  - Graft vs Host Disease/*immunology
MH  - Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Stromal Cells/immunology
MH  - Transplantation, Homologous
EDAT- 2006/12/19 09:00
MHDA- 2007/02/03 09:00
CRDT- 2006/12/19 09:00
PHST- 2006/01/26 00:00 [received]
PHST- 2006/07/12 00:00 [revised]
PHST- 2006/08/17 00:00 [accepted]
PHST- 2006/12/19 09:00 [pubmed]
PHST- 2007/02/03 09:00 [medline]
PHST- 2006/12/19 09:00 [entrez]
AID - S0198-8859(06)00481-2 [pii]
AID - 10.1016/j.humimm.2006.08.298 [doi]
PST - ppublish
SO  - Hum Immunol. 2006 Dec;67(12):976-85. doi: 10.1016/j.humimm.2006.08.298. Epub 2006 
      Oct 18.