PMID- 17175294
OWN - NLM
STAT- MEDLINE
DCOM- 20070430
LR  - 20061218
IS  - 0041-1345 (Print)
IS  - 0041-1345 (Linking)
VI  - 38
IP  - 10
DP  - 2006 Dec
TI  - Does endothelial chimerism correlate with renal allograft rejection?
PG  - 3430-3
AB  - BACKGROUND: The blood vessels of a transplanted organ are an interface between 
      the donor and the recipient. The endothelium is believed to be a major target for 
      graft rejection. After transplantation endothelial cells of a transplanted organ 
      may be of recipient origin. OBJECTIVES: In this study we sought to determine 
      whether endothelial chimerism correlates with graft rejection. METHODS: Biopsy 
      samples from 34 renal transplants of female recipients who received kidneys from 
      male donors were studied for the presence of endothelial cells of recipient 
      origin. Formalin-fixed, paraffin-embedded tissue sections of renal biopsy samples 
      were examined by fluorescence in situ hybridization (FISH) for the presence of 
      endothelial cells containing two X chromosomes, using a biotinylated Y-chromosome 
      probe and digoxigenin-labeled X-chromosome probe. RESULTS: The FISH methods 
      identified endothelial cells of recipient origin. Endothelial chimerism was 
      common, irrespective of rejection. Its presence was focal with these elements, 
      coexisting in the biopsy. CONCLUSIONS: We observed no correlation between the 
      percentage of recipient endothelial cells among vascular elements and the type of 
      graft rejection (P > .05).
FAU - Bai, H W
AU  - Bai HW
AD  - Organ Transplantation Center of PLA, Second Affiliated Hospital of PLA General 
      Hospital, Beijing, China.
FAU - Shi, B Y
AU  - Shi BY
FAU - Qian, Y Y
AU  - Qian YY
FAU - Na, Y Q
AU  - Na YQ
FAU - Cai, M
AU  - Cai M
FAU - Zeng, X
AU  - Zeng X
FAU - Zhong, D R
AU  - Zhong DR
FAU - Wu, S F
AU  - Wu SF
FAU - Chang, J Y
AU  - Chang JY
FAU - Zhou, W Q
AU  - Zhou WQ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
SB  - IM
MH  - Biopsy
MH  - Chromosomes, Human, X
MH  - Chromosomes, Human, Y
MH  - Endothelium, Vascular/*immunology/pathology
MH  - Female
MH  - Graft Rejection/*immunology/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kidney Transplantation/*immunology/pathology
MH  - Lymphocytes/immunology/pathology
MH  - Male
MH  - *Transplantation Chimera
MH  - Transplantation, Homologous
EDAT- 2006/12/19 09:00
MHDA- 2007/05/01 09:00
CRDT- 2006/12/19 09:00
PHST- 2006/05/21 00:00 [received]
PHST- 2006/12/19 09:00 [pubmed]
PHST- 2007/05/01 09:00 [medline]
PHST- 2006/12/19 09:00 [entrez]
AID - S0041-1345(06)01399-6 [pii]
AID - 10.1016/j.transproceed.2006.10.154 [doi]
PST - ppublish
SO  - Transplant Proc. 2006 Dec;38(10):3430-3. doi: 10.1016/j.transproceed.2006.10.154.