PMID- 1717621
OWN - NLM
STAT- MEDLINE
DCOM- 19911112
LR  - 20131121
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 118
IP  - 3
DP  - 1991 Sep
TI  - A lavage method for dynamic intraarticular monitoring of animal joints in situ: 
      quantification and release kinetics of histamine after selective synovial mast 
      cell activation by diverse secretagogues.
PG  - 269-79
AB  - Evidence implicating synovial mast cells in the initiation and perpetuation of 
      arthritis has increased. We have developed a method of joint lavage to monitor 
      dynamic intraarticular events in the intact animal. Lavage was done before and 
      after immunologic (passive sensitization followed by intravenous specific antigen 
      or intraarticular anti-rat immunoglobulin E [IgE] heteroantiserum) and 
      nonimmunologic (intraarticular calcium ionophore A-23187; phorbol 12-myristate, 
      13-acetate; and compound 48/80) synovial mast cell activation. To quantify and 
      analyze synovial mast cell mediator release kinetics in situ, we measured lavage 
      fluid histamine. With all activation protocols except A-23187, histamine release 
      was evident within 5 minutes after introduction of the stimulus. The quantitative 
      and chronological similarities between immunologically induced and compound 
      48/80-induced synovial mast cell histamine release kinetics suggested that 
      connective tissue type mast cells are an important source of inflammatory 
      mediators in rat joints. We also measured joint lavage fluid histamine levels in 
      rats immunized with an active sensitization protocol. Histamine levels were 
      determined by the autoanalyzer method and were confirmed by using a commercially 
      available radioimmunoassay that uses a monoclonal antibody against acetylated 
      histamine. We found that in many of these animals, at the peak of the serum IgE 
      response, joint lavage fluid histamine levels were very high even before 
      challenge with specific antigen, and that this increase was not due to diffusion 
      into the joint of abnormally elevated plasma histamine. These data suggested that 
      synovial mast cells are preferentially activated in states of high serum IgE 
      immune responses. We have used a simple, inexpensive, rapid lavage technique to 
      generate the first data on histamine release kinetics after selective synovial 
      mast cell activation in the intact animal. The technique can be adapted for 
      investigation of release kinetics of a variety of other substances from activated 
      synovial cells and can be used in other arthritis models.
FAU - Malone, D G
AU  - Malone DG
AD  - Department of Medicine, University of Wisconsin.
FAU - Verbsky, J W
AU  - Verbsky JW
FAU - Dolan, P W
AU  - Dolan PW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Antigens)
RN  - 0 (Immune Sera)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 4091-50-3 (p-Methoxy-N-methylphenethylamine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Antigens/immunology
MH  - Calcimycin/pharmacology
MH  - Capillary Permeability
MH  - *Histamine Release
MH  - Immune Sera/immunology
MH  - Immunization
MH  - Immunoglobulin E/immunology
MH  - *Joints
MH  - Male
MH  - Mast Cells/*metabolism/physiology
MH  - Monitoring, Physiologic/*methods
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Synovial Membrane/blood supply/cytology/*metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Therapeutic Irrigation/*methods
MH  - p-Methoxy-N-methylphenethylamine/pharmacology
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
AID - 0022-2143(91)90071-E [pii]
PST - ppublish
SO  - J Lab Clin Med. 1991 Sep;118(3):269-79.