PMID- 17176751
OWN - NLM
STAT- MEDLINE
DCOM- 20070130
LR  - 20190922
IS  - 0920-5063 (Print)
IS  - 0920-5063 (Linking)
VI  - 17
IP  - 11
DP  - 2006
TI  - Antimicrobial polypeptide multilayer nanocoatings.
PG  - 1301-15
AB  - A multilayer coating (or film) of nanometer-thick layers can be made by 
      sequential adsorption of oppositely charged polyelectrolytes on a solid support. 
      The method is known as layer-by-layer assembly (LBL). No special apparatus is 
      required for LBL and nanofilms can be prepared under mild, physiological 
      conditions. A multilayer nanofilm in which at least one of the constituent 
      species is a polypeptide is a polypeptide multilayer nanofilm. The present work 
      was aimed at assessing whether polypeptide multilayer nanofilms with specific 
      antimicrobial properties could be prepared by incorporation of a known 
      antimicrobial agent in the film structure, in this case the edible protein hen 
      egg white lysozyme (HEWL). The chicken enzyme is widely employed as a human food 
      preservative. An advantage of LBL in this context is that the nanofilm is 
      fabricated directly on the surface of interest, eliminating the need to 
      incorporate the antimicrobial in other packaging materials. Here, nanofilms were 
      made of poly(L-glutamic acid) (PLGA), which is highly negatively charged in the 
      mildly acidic pH range, and HEWL, which has a high net positive charge at acidic 
      pH. We show that PLGA/HEWL nanofilms inhibit growth of the model microbe 
      Microccocus luteus in the surrounding liquid medium. The amount of HEWL released 
      from PLGA/HEWL films depends on the number of HEWL layers and therefore on the 
      total quantity of HEWL in the films. This initial study provides a sketch of the 
      scope for further development of LBL in the area of antimicrobial polypeptide 
      multilayer films. Potential applications of such films include strategies for 
      food preservation and coatings for implant devices.
FAU - Rudra, Jai S
AU  - Rudra JS
AD  - Biomedical Engineering, Bionanosystems Engineering Laboratory, Center for Applied 
      Physics Studies, PO Box 10348, Louisiana Tech University, Ruston, LA 71272, USA.
FAU - Dave, Komal
AU  - Dave K
FAU - Haynie, Donald T
AU  - Haynie DT
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Biomater Sci Polym Ed
JT  - Journal of biomaterials science. Polymer edition
JID - 9007393
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Peptides)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Anti-Bacterial Agents/*chemical synthesis/pharmacology
MH  - Anti-Infective Agents/*chemical synthesis
MH  - Lactic Acid
MH  - Micrococcus luteus/drug effects
MH  - *Nanostructures
MH  - Peptides/chemical synthesis
MH  - Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers
EDAT- 2006/12/21 09:00
MHDA- 2007/01/31 09:00
CRDT- 2006/12/21 09:00
PHST- 2006/12/21 09:00 [pubmed]
PHST- 2007/01/31 09:00 [medline]
PHST- 2006/12/21 09:00 [entrez]
AID - 10.1163/156856206778667433 [doi]
PST - ppublish
SO  - J Biomater Sci Polym Ed. 2006;17(11):1301-15. doi: 10.1163/156856206778667433.