PMID- 17177169
OWN - NLM
STAT- MEDLINE
DCOM- 20070302
LR  - 20191210
IS  - 0260-437X (Print)
IS  - 0260-437X (Linking)
VI  - 27
IP  - 1
DP  - 2007 Jan-Feb
TI  - High-performance liquid chromatography coupled with electrospray ionization 
      tandem mass spectrometry for the determination of flocoumafen and brodifacoum in 
      whole blood.
PG  - 18-24
AB  - A high-performance liquid chromatographic-tandem mass spectrometric (HPLC-MS-MS) 
      assay was developed and validated to determine quantitatively flocoumafen and 
      brodifacoum in whole blood using warfarin as an internal standard (IS). 
      Liquid-liquid extraction, using ethyl acetate, was used to isolate flocoumafen, 
      brodifacoum and the IS from the biological matrix. Detection was performed on a 
      mass spectrometer by negative electrospray ionization (ESI) in multiple reaction 
      monitoring (MRM) mode. The calibration curves were linear (r(2) > 0.998) in the 
      concentration range of 0.1-100.0 ng ml(-1) with a lower limit of quantification 
      of 0.05 ng ml(-1) for flocoumafen, and 0.1 ng ml(-1) for brodifacoum in whole 
      blood. Intra-day and inter-day relative standard deviations (RSDs) were less than 
      8.0% and 10.8%, respectively. Recoveries of flocoumafen and brodifacoum ranged 
      from 78.0% to 83.7%. This assay can be used to determine trace flocoumafen and 
      brodifacoum in whole blood to investigate suspected poisoning of human and 
      animals.
CI  - Copyright 2006 John Wiley & Sons, Ltd.
FAU - Jin, Mi-Cong
AU  - Jin MC
AD  - Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China. 
      jmcjc@nbip.net
FAU - OuYang, Xiao-Kun
AU  - OuYang XK
FAU - Chen, Xiao-hong
AU  - Chen XH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (4-Hydroxycoumarins)
RN  - 0 (Anticoagulants)
RN  - 2Z80062XQ4 (flocoumafen)
RN  - 5Q7ZVV76EI (Warfarin)
RN  - A25P3CP5S7 (bromfenacoum)
SB  - IM
MH  - 4-Hydroxycoumarins/*blood/chemistry
MH  - Anticoagulants/*blood/chemistry
MH  - Chromatography, High Pressure Liquid/*methods/standards
MH  - Drug Monitoring/methods
MH  - Drug Stability
MH  - Forensic Toxicology/methods
MH  - Humans
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Electrospray Ionization/*methods/standards
MH  - Tandem Mass Spectrometry/*methods/standards
MH  - Warfarin/blood
EDAT- 2006/12/21 09:00
MHDA- 2007/03/03 09:00
CRDT- 2006/12/21 09:00
PHST- 2006/12/21 09:00 [pubmed]
PHST- 2007/03/03 09:00 [medline]
PHST- 2006/12/21 09:00 [entrez]
AID - 10.1002/jat.1179 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2007 Jan-Feb;27(1):18-24. doi: 10.1002/jat.1179.