PMID- 17177258
OWN - NLM
STAT- MEDLINE
DCOM- 20070222
LR  - 20220317
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 500
IP  - 5
DP  - 2007 Feb 10
TI  - Localization and comparative analysis of acid-sensing ion channel (ASIC1, 2, and 
      3) mRNA expression in mouse colonic sensory neurons within thoracolumbar dorsal 
      root ganglia.
PG  - 863-75
AB  - Reducing colonic mechanosensitivity is an important potential strategy for 
      reducing visceral pain. Mice lacking acid-sensing ion channels (ASIC) 1, 2, and 3 
      show altered colonic mechanosensory function, implicating ASICs in the 
      mechanotransduction process. Deletion of ASICs affects mechanotransduction in 
      visceral and cutaneous afferents differently, suggesting differential expression. 
      We determined relative expression of ASIC1, 2, and 3 in mouse thoracolumbar 
      dorsal root ganglia (DRG) by quantitative reverse-transcriptase polymerase chain 
      reaction (RT-PCR) analysis (QPCR) and specifically in retrogradely traced colonic 
      neurons isolated via laser capture microdissection. Localization of ASIC 
      expression in DRG was determined with fluorescence in situ hybridization (FISH) 
      and retrograde tracing. QPCR of whole thoracolumbar DRG revealed and abundance of 
      ASIC2 > ASIC1 > ASIC3. Similarly, FISH of all neurons in thoracolumbar DRG 
      demonstrated that ASIC2 was expressed in the most (40 +/- 1%) neurons, followed 
      by ASIC3 (24 +/- 1%), then ASIC1 (18 +/- 1%). Retrograde tracing from the distal 
      colon labeled 4 +/- 1% of neurons in T10-L1 DRG. In contrast to whole DRG, FISH 
      of colonic neurons showed ASIC3 expression in 73 +/- 2%, ASIC2 in 47 +/- 0.5%, 
      and ASIC1 in 30 +/- 2%. QPCR of laser captured colonic neurons revealed that 
      ASIC3 was the most abundant ASIC transcript, followed by ASIC1, then ASIC2. We 
      conclude that ASIC1, 2, and 3 are expressed preferentially in colonic neurons 
      within thoracolumbar DRG. In particular ASIC3, the least abundant in the general 
      population, is the most abundant ASIC transcript in colonic neurons. The 
      prevalence of ASIC3 in neurons innervating the colon supports 
      electrophysiological data showing that it makes a major contribution to colonic 
      mechanotransduction and therefore may be a target for the treatment of visceral 
      pain.
CI  - 2006 Wiley-Liss, Inc.
FAU - Hughes, Patrick A
AU  - Hughes PA
AD  - Nerve-Gut Research Laboratory, Department of Gastroenterology, Hepatology and 
      General Medicine, Royal Adelaide Hospital, Adelaide, South Australia, 5000, 
      Australia.
FAU - Brierley, Stuart M
AU  - Brierley SM
FAU - Young, Richard L
AU  - Young RL
FAU - Blackshaw, L Ashley
AU  - Blackshaw LA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (ASIC1 protein, mouse)
RN  - 0 (ASIC2 protein, mouse)
RN  - 0 (ASIC3 protein, mouse)
RN  - 0 (Acid Sensing Ion Channels)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sodium Channels)
SB  - IM
MH  - Acid Sensing Ion Channels
MH  - Animals
MH  - Colon/*innervation
MH  - Female
MH  - Ganglia, Spinal/cytology/*metabolism
MH  - Lumbar Vertebrae
MH  - Male
MH  - Membrane Proteins/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Neurons, Afferent/*metabolism
MH  - Organ Specificity
MH  - RNA, Messenger/analysis
MH  - Sodium Channels/genetics/*metabolism
MH  - Thoracic Vertebrae
MH  - Tissue Distribution
EDAT- 2006/12/21 09:00
MHDA- 2007/02/23 09:00
CRDT- 2006/12/21 09:00
PHST- 2006/12/21 09:00 [pubmed]
PHST- 2007/02/23 09:00 [medline]
PHST- 2006/12/21 09:00 [entrez]
AID - 10.1002/cne.21204 [doi]
PST - ppublish
SO  - J Comp Neurol. 2007 Feb 10;500(5):863-75. doi: 10.1002/cne.21204.