PMID- 17177330
OWN - NLM
STAT- MEDLINE
DCOM- 20080505
LR  - 20121115
IS  - 1008-9292 (Print)
IS  - 1008-9292 (Linking)
VI  - 35
IP  - 6
DP  - 2006 Nov
TI  - [Effect of recombinant beta-defensin-2 peptide on apoptosis of pulmonary tissue 
      in rats with sepsis].
PG  - 600-4
AB  - OBJECTIVE: To investigate the effect of recombinant beta-defensin 2 (BD-2) on the 
      apoptosis of pulmonary tissue in rats with sepsis. METHODS: Forty-eight SD rats 
      were randomly divided into defensin group and controls. In control group 24 rats 
      received 10(7)PFU adenovirus via trachea intubation. In defensin group 24 rats 
      received 10(7)PFU recombinant adenovirus carrying all expression cassette of rat 
      BD-2 (Ad-rBD2). All rats received cecal ligation and puncture (CLP) to induce 
      sepsis 48 h following the administration of adenovirus. Rats of both groups were 
      sacrificed at 0, 12, 36 and 72 h after CLP; lungs were removed and fixed for 
      Haematoxylin and Eosin (HE) stain. Apoptosis was determined by terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) technique. 
      RESULT: The apoptosis index (AI) of lung cells increased significantly following 
      CLP in control group,while it was significantly lower in defensin group than that 
      of control group (P<0.05). In addition, a significant alveolar damage, 
      interstitial edema, and infiltration of inflammatory cells were observed in 
      control lungs, while it was less severe in defensin group. CONCLUSION: 
      Recombinant beta-defensin 2 may reduce the apoptosis of lung cells and attenuate 
      lung injury.
FAU - Xu, Xiao-yi
AU  - Xu XY
AD  - Department of Anesthesiology, Sir Run Run Shaw Hospital, College of Medicine, 
      Zhejiang University, Hangzhou 310016, China.
FAU - Shi, Zhuo
AU  - Shi Z
FAU - Bao, Jun-ming
AU  - Bao JM
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhejiang Da Xue Xue Bao Yi Xue Ban
JT  - Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical 
      sciences
JID - 100927946
RN  - 0 (DEFB4A protein, human)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (beta-Defensins)
SB  - IM
MH  - Adenoviridae/genetics/growth & development
MH  - Animals
MH  - Apoptosis/genetics/*physiology
MH  - Genetic Therapy/methods
MH  - Genetic Vectors
MH  - Humans
MH  - Lung Diseases/pathology/therapy/virology
MH  - Male
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recombinant Fusion Proteins/genetics/*physiology
MH  - Viremia/*therapy/virology
MH  - beta-Defensins/genetics/*physiology
EDAT- 2006/12/21 09:00
MHDA- 2008/05/06 09:00
CRDT- 2006/12/21 09:00
PHST- 2006/12/21 09:00 [pubmed]
PHST- 2008/05/06 09:00 [medline]
PHST- 2006/12/21 09:00 [entrez]
PST - ppublish
SO  - Zhejiang Da Xue Xue Bao Yi Xue Ban. 2006 Nov;35(6):600-4.