PMID- 17177488
OWN - NLM
STAT- MEDLINE
DCOM- 20070316
LR  - 20061220
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 54
IP  - 26
DP  - 2006 Dec 27
TI  - Screening of tetracycline residues in fish muscles by CCD camera-based 
      solid-surface fluorescence.
PG  - 9687-95
AB  - A methodology for the screening of tetracyclines (TCs), including tetracycline 
      (TC), oxytetracycline (OTC), and chlorotetracycline (CTC), in different fish 
      muscle matrices has been proposed. This method was based on in situ fluorescent 
      derivation of TCs, transferring weakly fluorescing TCs to highly fluorescent 
      species, on alkaline-activated solid silica gel G plates (SGGPs). By coupling 
      solid-surface fluorescence (SSF) with charge-coupled device (CCD) camera imaging, 
      a CCD camera-based SSF (CCD-SSF) methodology has been developed. Calibration 
      curve, repeatability, selectivity, limit of detection (LOD), and limit of 
      quantification (LOQ) have been explored for evaluating the performance of the 
      method itself. Linear calibration curves were obtained over a range of 0.20-1.0 
      ng/spot for all three TCs. The LODs, defined as 3sigma, for TC, OTC, and CTC were 
      0.14, 0.15, and 0.16 ng/spot, respectively. The trueness of method was validated 
      by HPLC, and no significant difference between CCD-SSF and HPLC was found, on a 
      basis of 95% confidence level. By spiked recovery studies, a linear calibration 
      curve ranging from 20 to 300 microg/kg of TC in fish muscle samples with a 
      correlation coefficient (R 2) equal to 0.994 was obtained. The total average 
      recovery for TC in fish muscle samples from six different fish matrices, 
      fortified with TC at 50, 100, and 200 microg/kg levels, was 75.7% with average 
      relative standard deviations (RSDs) ranging from 2.0 to 7.7%. RSDs ranged from 
      2.5 to 5.8% and from 5.2 to 7.6% for in-day and interday repeatability, 
      respectively. The detection and quantification limits in fish muscle matrices 
      were 16 and 53 microg/kg of TCs, respectively. The newly developed CCD-SSF method 
      has been applied to the screening of the TC residues in fish muscle samples. The 
      method has been demonstrated to bear some advantages, such as its simplicity, 
      high throughput, low cost, use of fewer pollutants, and reasonable sensitivity.
FAU - Sun, Xiao-Ying
AU  - Sun XY
AD  - The MOE Key Laboratory of Analytical Science and the Key Laboratory for Chemical 
      Biology of Fujian Province, Department of Chemistry, College of Chemistry and 
      Chemical Engineering, Xiamen University, Xiamen 361005, China.
FAU - Chen, Hao
AU  - Chen H
FAU - Gao, Hong
AU  - Gao H
FAU - Guo, Xiang-Qun
AU  - Guo XQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Tetracyclines)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - *Fishes
MH  - *Fluorescence
MH  - Muscles/*chemistry
MH  - Quality Control
MH  - Sensitivity and Specificity
MH  - Spectrometry, Fluorescence
MH  - Tetracyclines/*analysis
EDAT- 2006/12/21 09:00
MHDA- 2007/03/17 09:00
CRDT- 2006/12/21 09:00
PHST- 2006/12/21 09:00 [pubmed]
PHST- 2007/03/17 09:00 [medline]
PHST- 2006/12/21 09:00 [entrez]
AID - 10.1021/jf0622580 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2006 Dec 27;54(26):9687-95. doi: 10.1021/jf0622580.