PMID- 1717797
OWN - NLM
STAT- MEDLINE
DCOM- 19911112
LR  - 20190827
IS  - 0165-0270 (Print)
IS  - 0165-0270 (Linking)
VI  - 38
IP  - 1
DP  - 1991 Jun
TI  - Applications of piezoelectric fluid jetting devices to neuroscience research.
PG  - 81-8
AB  - Piezoelectric pumps or "jets" are used in industry for precise dispensing of 
      small volumes of fluids. In the present work we have tested the feasibility of 
      using these piezoelectric devices for dispensing fluids in neurobiological 
      research. In one experiment, 70 picoliter (pl) droplets of histochemical reagent 
      were jetted onto discrete targets of frozen tissue sections, and qualitative and 
      quantitative histochemical studies were done on the small (170 microns diameter) 
      circle of tissue wet by the droplets. In the second experiment, 70 pl droplets of 
      neuroactive drugs were jetted onto brain tissue slices while recording single 
      neurons extracellularly in vitro, and the effects of the drugs were found to vary 
      systematically as a function of the number of drops and the distance between drop 
      application and the recorded neuron. The results indicate that piezoelectric jets 
      could have wide application for dispensing fluids in neurobiological research.
FAU - Bernardini, G L
AU  - Bernardini GL
AD  - Laboratory of Neurobiology, University of Texas, Dallas, Richardson 75080.
FAU - Rampy, B A
AU  - Rampy BA
FAU - Howell, G A
AU  - Howell GA
FAU - Hayes, D J
AU  - Hayes DJ
FAU - Frederickson, C J
AU  - Frederickson CJ
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Neurosci Methods
JT  - Journal of neuroscience methods
JID - 7905558
RN  - 0 (Glutamates)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - *Administration, Topical
MH  - Animals
MH  - Brain/cytology/physiology
MH  - Dopamine/administration & dosage
MH  - Electrophysiology
MH  - Equipment Design
MH  - Glutamates/administration & dosage
MH  - Glutamic Acid
MH  - Mice
MH  - Neurons/physiology
MH  - Neurosciences/*instrumentation
MH  - Research
MH  - Staining and Labeling
EDAT- 1991/06/01 00:00
MHDA- 1991/06/01 00:01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 1991/06/01 00:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
AID - 0165-0270(91)90157-U [pii]
AID - 10.1016/0165-0270(91)90157-u [doi]
PST - ppublish
SO  - J Neurosci Methods. 1991 Jun;38(1):81-8. doi: 10.1016/0165-0270(91)90157-u.