PMID- 17177971
OWN - NLM
STAT- MEDLINE
DCOM- 20070329
LR  - 20181113
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 147
IP  - 1
DP  - 2007 Jan
TI  - Spreading of antibody reactivity to non-thyroid antigens during experimental 
      immunization with human thyroglobulin.
PG  - 120-7
AB  - Intermolecular spreading of antibody reactivity has been implicated in the 
      evolution of autoimmune disease. In this study, spreading of antibody reactivity 
      to non-thyroid autoantigens after experimental immunization with thyroglobulin 
      (Tg) was investigated. For this purpose, two rabbits were injected with human Tg 
      six times (stages 1-6) every 3 weeks. Animals were also bled before priming. 
      Antisera were tested by enzyme-linked immunosorbent assay (ELISA) for reactivity 
      to several non-thyroid antigens: bovine serum albumin (BSA), native DNA (nDNA), 
      human myosin, human globular (G) and filamentous (F) actin and porcine tubulin. 
      Tg-immunized animals developed the following serological reactivity pattern: (a) 
      high reactivity to myosin from stage 2 onward, (b) significant reactivity to 
      F-actin, remaining high up to stage 6, (c) reactivity to BSA with a peak at stage 
      3, (d) a small increase of reactivity to G-actin at stage 3 and (e) no increase 
      of reactivity to nDNA and tubulin. The study of affinity-purified anti-Tg 
      antibodies and the use of competitive assays revealed that reactivity to F-actin 
      was not due to cross-reaction with Tg. On the contrary, reactivity to myosin 
      during the first stages of immunization was due to cross-reaction with Tg, while 
      at stage 6 it became myosin-specific. Reactivity to BSA at stage 3 was also due 
      to cross-reaction with Tg. We conclude that at least part of the induced anti-Tg 
      antibodies may result from the expansion of B cell clones producing polyreactive 
      natural autoantibodies, and polyreactivity of anti-Tg antibodies during the first 
      stages of Tg-immunization may be responsible for the intermolecular spreading of 
      antibody response.
FAU - Thrasyvoulides, A
AU  - Thrasyvoulides A
AD  - Laboratory of Immunology, Department of Biochemistry, Hellenic Pasteur Institute, 
      Athens, Greece.
FAU - Liakata, E
AU  - Liakata E
FAU - Lymberi, P
AU  - Lymberi P
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Actins)
RN  - 0 (Antibodies)
RN  - 0 (Antigens)
RN  - 0 (Autoantibodies)
RN  - 0 (Epitopes)
RN  - 0 (anti-thyroglobulin)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 9010-34-8 (Thyroglobulin)
RN  - EC 3.6.4.1 (Myosins)
SB  - IM
MH  - Actins/immunology
MH  - Animals
MH  - Antibodies/*blood
MH  - Antibody Formation
MH  - Antibody Specificity
MH  - Antigens/*immunology
MH  - Autoantibodies/blood
MH  - Cross Reactions
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Epitopes
MH  - Humans
MH  - Immunization/methods
MH  - Immunosorbent Techniques
MH  - Myosins/immunology
MH  - Protein Conformation
MH  - Rabbits
MH  - Serum Albumin, Bovine/immunology
MH  - Thyroglobulin/*pharmacology
PMC - PMC1810443
EDAT- 2006/12/21 09:00
MHDA- 2007/03/30 09:00
PMCR- 2008/01/01
CRDT- 2006/12/21 09:00
PHST- 2006/12/21 09:00 [pubmed]
PHST- 2007/03/30 09:00 [medline]
PHST- 2006/12/21 09:00 [entrez]
PHST- 2008/01/01 00:00 [pmc-release]
AID - CEI3246 [pii]
AID - 10.1111/j.1365-2249.2006.03246.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2007 Jan;147(1):120-7. doi: 10.1111/j.1365-2249.2006.03246.x.