PMID- 1717805
OWN - NLM
STAT- MEDLINE
DCOM- 19911113
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 49
IP  - 18
DP  - 1991
TI  - Two-dimensional gel electrophoretic profile of rat basophilic leukemia (RBL) and 
      mast cells.
PG  - 1289-94
AB  - RBL cells are not differentiated, but resemble mucosal mast cells (MMC). 
      Two-dimensional (2-D) gel electrophoresis following isoelectric focusing (IEF) 
      was performed using purified rat peritoneal mast cells and RBL cells. Certain 
      similarities were identified with silver staining between mast cells and 
      stationary phase (72 hr) RBL cells. RBL cells were also labelled with 
      [35S]-cysteine in order to study the specific expression of proteins during 
      logarithmic or stationary growth phases. Only stationary phase RBL cells appeared 
      to specifically express three proteins of 42, 55 and 93 kD and were still capable 
      of secreting histamine in response to immunoglobulin E (IgE) and specific 
      antigen. These results suggest that specific RBL cell proteins may be used as 
      markers for further analysis of their maturation/differentiation.
FAU - Kouretas, D
AU  - Kouretas D
AD  - Department of Pharmacology, Tufts University School of Medicine, Boston, MA 
      02111.
FAU - Trnovsky, J
AU  - Trnovsky J
FAU - Holdridge, M
AU  - Holdridge M
FAU - Theoharides, T C
AU  - Theoharides TC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Cell Cycle/physiology
MH  - Cells, Cultured
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Histamine Release/physiology
MH  - Immunoglobulin E/metabolism
MH  - Isoelectric Focusing
MH  - Leukemia, Basophilic, Acute/*metabolism/pathology
MH  - Male
MH  - Mast Cells/*chemistry/cytology/metabolism
MH  - Neoplasm Proteins/*chemistry
MH  - Proteins/*chemistry
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Silver Staining
MH  - Tumor Cells, Cultured
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 0024-3205(91)90192-E [pii]
AID - 10.1016/0024-3205(91)90192-e [doi]
PST - ppublish
SO  - Life Sci. 1991;49(18):1289-94. doi: 10.1016/0024-3205(91)90192-e.