PMID- 17178104
OWN - NLM
STAT- MEDLINE
DCOM- 20070305
LR  - 20070103
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 353
IP  - 2
DP  - 2007 Feb 9
TI  - Simple screening method for differentially methylated regions of the genome using 
      a small number of cells.
PG  - 275-9
AB  - Genomic DNA methylation is a major epigenetic mechanism controlling the 
      expression of genetic information. Therefore, identifying regions of the genome 
      that are differentially methylated in different cells is a useful strategy for 
      the study of biological phenomena. To date, several useful screening methods have 
      been established for identifying differentially methylated genomic regions. 
      However, it is impossible to use these methods in fields of study in which it is 
      difficult to obtain a large number of uniform cells, because considerable amounts 
      of genomic DNA are required. Given this situation, we developed a method for 
      preparing large genomic DNA from a small number of cells, and a simple and highly 
      sensitive method for screening for differentially methylated sites. Combined, 
      these two methods comprise a simple screening method, which we named 
      "Differential Methylation Site Scanning" (DMSS), for identifying differentially 
      methylated regions of the genome from a small number of cells. Just 10 cells are 
      sufficient for the method described here.
FAU - Hamada, Tsuyoshi
AU  - Hamada T
AD  - Department of Anatomy and Neurobiology, National Defense Medical Collage, 3-2 
      Namiki, Tokorozawa, Saitama 359-8513, Japan. thamada@ndmc.ac.jp
FAU - Murasawa, Satoshi
AU  - Murasawa S
FAU - Asahara, Takayuki
AU  - Asahara T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061212
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cell Line
MH  - Chromosome Mapping/*methods
MH  - DNA/*genetics
MH  - DNA Fingerprinting/*methods
MH  - *DNA Methylation
MH  - HeLa Cells
MH  - Humans
MH  - Kidney/cytology/*physiology
MH  - Polymerase Chain Reaction/*methods
MH  - Sequence Analysis, DNA/*methods
EDAT- 2006/12/21 09:00
MHDA- 2007/03/06 09:00
CRDT- 2006/12/21 09:00
PHST- 2006/11/21 00:00 [received]
PHST- 2006/12/04 00:00 [accepted]
PHST- 2006/12/21 09:00 [pubmed]
PHST- 2007/03/06 09:00 [medline]
PHST- 2006/12/21 09:00 [entrez]
AID - S0006-291X(06)02645-3 [pii]
AID - 10.1016/j.bbrc.2006.12.024 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2007 Feb 9;353(2):275-9. doi: 
      10.1016/j.bbrc.2006.12.024. Epub 2006 Dec 12.