PMID- 17178627
OWN - NLM
STAT- MEDLINE
DCOM- 20070220
LR  - 20061220
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 82
IP  - 12
DP  - 2006 Dec
TI  - Analysis of gamma-rays induced chromosome aberrations: a fingerprint evaluation 
      with a combination of pan-centromeric and pan-telomeric probes.
PG  - 869-75
AB  - PURPOSE: To evaluate the types of induced chromosome aberrations after the 
      exposure of peripheral blood to gamma-rays by the simultaneous detection of all 
      centromeres and telomeres; and to analyse the suitability of different radiation 
      fingerprints for the assessment of radiation quality in cases of recent 
      exposures. MATERIAL AND METHODS: Peripheral blood samples were irradiated at 2, 4 
      and 6 Gy of gamma-rays. Cytogenetic analysis was carried out by fluorescence in 
      situ hybridization (FISH) technique with pan-centromeric and peptide nucleic acid 
      (PNA)-telomeric DNA probes. Cells were analysed using a Cytovision FISH 
      workstation, chromosome aberrations and the length of the acentric fragments were 
      recorded. RESULTS: The total number of the incomplete chromosome elements was 
      276. The ratio between incomplete elements and multicentrics was 0.38. The number 
      of acentrics was 1096, 71% were complete acentrics, 15% incomplete acentrics, and 
      14% interstitial fragments. The relative length of complete, incomplete and 
      interstitial acentrics fragments were 2.70 +/- 0.04, 1.91 +/- 0.07, and 1.42 +/- 
      0.04 respectively. The mean value of the F-ratio was 11.5 higher than the one, 
      5.5, previously obtained for alpha-particles. For the G-ratio there was no 
      difference between gamma-rays and alpha-particles, 2.8 and 2.8 respectively. The 
      mean value of the H-ratio for gamma-rays, 0.25, was lower than for 
      alpha-particles 0.40. CONCLUSION: The results support that the percentage of 
      incomplete chromosome aberrations depends on radiation type; low-linear energy 
      transfer (LET) radiation would produces less incomplete aberrations than high-LET 
      radiation. The F- and H-ratios seem to be good indicators of radiation quality, 
      although a real estimation of the H-ratio is only possible using pan-telomeric 
      probes.
FAU - Benkhaled, L
AU  - Benkhaled L
AD  - Unitat d'Antropologia, Dpt. Biologia Animal, Biologia Vegetal i Ecologia, 
      Facultat de Ciencies, Universitat Autonoma de Barcelona, Bellaterra, Spain.
FAU - Barrios, L
AU  - Barrios L
FAU - Mestres, M
AU  - Mestres M
FAU - Caballin, M R
AU  - Caballin MR
FAU - Ribas, M
AU  - Ribas M
FAU - Barquinero, J F
AU  - Barquinero JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adult
MH  - Centromere/genetics/radiation effects
MH  - Chromosome Aberrations/*radiation effects
MH  - Chromosomes/*radiation effects
MH  - DNA Fingerprinting/*methods
MH  - DNA Probes/*genetics
MH  - Dose-Response Relationship, Radiation
MH  - *Gamma Rays
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Monocytes/*radiation effects
MH  - Radiation Dosage
MH  - Telomere/genetics/radiation effects
EDAT- 2006/12/21 09:00
MHDA- 2007/02/21 09:00
CRDT- 2006/12/21 09:00
PHST- 2006/12/21 09:00 [pubmed]
PHST- 2007/02/21 09:00 [medline]
PHST- 2006/12/21 09:00 [entrez]
AID - H62L40424584H205 [pii]
AID - 10.1080/09553000600979092 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 2006 Dec;82(12):869-75. doi: 10.1080/09553000600979092.