PMID- 17178913
OWN - NLM
STAT- MEDLINE
DCOM- 20070201
LR  - 20181113
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 175
IP  - 6
DP  - 2006 Dec 18
TI  - The nuclear receptor hepatocyte nuclear factor 4alpha acts as a morphogen to 
      induce the formation of microvilli.
PG  - 971-80
AB  - Microvilli are actin-based organelles found on apical plasma membranes that are 
      involved in nutrient uptake and signal transduction. Numerous components, 
      including ezrin/radixin/moesin (ERM) proteins, have been identified that link 
      filamentous actins to transmembrane proteins, but the signals driving microvillus 
      biogenesis are not known. In this study, we show that the conditional and/or 
      ectopic expression of a nuclear receptor, hepatocyte nuclear factor 4alpha 
      (HNF4alpha), triggers microvillus morphogenesis. We also demonstrate that 
      HNF4alpha expression induces ERM-binding phosphoprotein 50 (EBP50) expression and 
      that attenuation of EBP50 using RNA interference inhibits microvillus 
      development. We conclude that HNF4alpha acts as a morphogen to trigger 
      microvillus formation.
FAU - Chiba, Hideki
AU  - Chiba H
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo 
      060-8556, Japan. hidchiba@sapmed.ac.jp
FAU - Sakai, Naoyuki
AU  - Sakai N
FAU - Murata, Masaki
AU  - Murata M
FAU - Osanai, Makoto
AU  - Osanai M
FAU - Ninomiya, Takafumi
AU  - Ninomiya T
FAU - Kojima, Takashi
AU  - Kojima T
FAU - Sawada, Norimasa
AU  - Sawada N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Hepatocyte Nuclear Factor 4)
RN  - 0 (Hnf4a protein, rat)
RN  - 0 (Membrane Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Retinoid X Receptor alpha)
RN  - 0 (Retinoid X Receptor gamma)
RN  - 0 (Sodium-Hydrogen Exchangers)
RN  - 0 (ezrin)
RN  - 0 (sodium-hydrogen exchanger regulatory factor)
RN  - 144131-77-1 (moesin)
RN  - 144517-21-5 (radixin)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Animals
MH  - Cells, Cultured/drug effects/metabolism
MH  - Cytoskeletal Proteins/metabolism
MH  - Endothelium, Vascular/cytology/metabolism
MH  - Hepatocyte Nuclear Factor 4/genetics/*metabolism
MH  - Immunoblotting
MH  - Immunoenzyme Techniques
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Microfilament Proteins/metabolism
MH  - Microvilli/*metabolism
MH  - *Morphogenesis
MH  - Neoplastic Stem Cells/drug effects/metabolism
MH  - Phosphoproteins/antagonists & inhibitors/genetics/metabolism
MH  - Phosphorylation
MH  - Protein Binding
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Small Interfering/pharmacology
MH  - Rats
MH  - Retinoid X Receptor alpha/metabolism
MH  - Retinoid X Receptor gamma/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sodium-Hydrogen Exchangers/antagonists & inhibitors/genetics/metabolism
MH  - Tretinoin/pharmacology
PMC - PMC2064706
EDAT- 2006/12/21 09:00
MHDA- 2007/02/03 09:00
PMCR- 2007/06/18
CRDT- 2006/12/21 09:00
PHST- 2006/12/21 09:00 [pubmed]
PHST- 2007/02/03 09:00 [medline]
PHST- 2006/12/21 09:00 [entrez]
PHST- 2007/06/18 00:00 [pmc-release]
AID - jcb.200608012 [pii]
AID - 200608012 [pii]
AID - 10.1083/jcb.200608012 [doi]
PST - ppublish
SO  - J Cell Biol. 2006 Dec 18;175(6):971-80. doi: 10.1083/jcb.200608012.