PMID- 17179222
OWN - NLM
STAT- MEDLINE
DCOM- 20070523
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 109
IP  - 8
DP  - 2007 Apr 15
TI  - Influence of ERK activation on decreased chemotaxis of mature human cord blood 
      monocyte-derived dendritic cells to CCL19 and CXCL12.
PG  - 3173-6
AB  - Dendritic cells (DCs) are important regulators in graft-versus-host disease 
      (GVHD). To gain insight into cord blood (CB) DC immunology, we compared 
      chemotactic responses of mature monocyte-derived DCs and maturation agent 
      lipopolysaccharide (LPS)-induced signaling between CB and adult blood (AB). 
      Mature CB DCs expressed reduced CCR7, but increased CXCR4. This was associated 
      with reduced migratory efficiency toward both CCR7 ligand CCL19 and CXCR4 ligand 
      CXCL12. LPS induced higher extracellular signal-regulated kinase (ERK) 
      phosphorylation in CB than in AB DCs. Specific inhibition of ERK during CB DC 
      maturation enhanced LPS-induced up-regulation of CCR7 and CXCR4 on CB DCs and 
      their chemotaxis toward CCL19 and CXCL12, to a level similar to that of mature AB 
      DCs. Overall, monocyte-derived CB DCs responded to LPS with stronger and 
      sustained ERK activation, which negatively correlated with LPS-induced 
      up-regulation of CCR7 and CXCR4 on CB DCs and their migratory responses. These 
      findings may have potential relevance to better understanding DC function in CB 
      transplantation.
FAU - Li, Geling
AU  - Li G
AD  - Department of Microbiology and Immunology, Walther Oncology Center, Indiana 
      University School of Medicine, 950 West Walnut Street, Indianapolis, IN 46202, 
      USA.
FAU - Basu, Sunanda
AU  - Basu S
FAU - Han, Myung-Kwan
AU  - Han MK
FAU - Kim, Young-June
AU  - Kim YJ
FAU - Broxmeyer, Hal E
AU  - Broxmeyer HE
LA  - eng
GR  - P01 HL 053586/HL/NHLBI NIH HHS/United States
GR  - R01 HL 56416/HL/NHLBI NIH HHS/United States
GR  - R01 HL 67384/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20061219
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (CCL19 protein, human)
RN  - 0 (CCR7 protein, human)
RN  - 0 (CXCL12 protein, human)
RN  - 0 (Chemokine CCL19)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Chemokines, CC)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, CCR7)
RN  - 0 (Receptors, CXCR4)
RN  - 0 (Receptors, Chemokine)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL19
MH  - Chemokine CXCL12
MH  - Chemokines, CC/*metabolism
MH  - Chemokines, CXC/*metabolism
MH  - Chemotaxis/drug effects/*physiology
MH  - Cord Blood Stem Cell Transplantation
MH  - Dendritic Cells/cytology/*metabolism
MH  - Enzyme Activation/drug effects/physiology
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Fetal Blood/cytology/*metabolism
MH  - Humans
MH  - Lipopolysaccharides/pharmacology
MH  - MAP Kinase Signaling System/drug effects/*physiology
MH  - Monocytes/cytology/*metabolism
MH  - Receptors, CCR7
MH  - Receptors, CXCR4/metabolism
MH  - Receptors, Chemokine/metabolism
PMC - PMC1852231
EDAT- 2006/12/21 09:00
MHDA- 2007/05/24 09:00
PMCR- 2008/04/15
CRDT- 2006/12/21 09:00
PHST- 2006/12/21 09:00 [pubmed]
PHST- 2007/05/24 09:00 [medline]
PHST- 2006/12/21 09:00 [entrez]
PHST- 2008/04/15 00:00 [pmc-release]
AID - S0006-4971(20)41686-6 [pii]
AID - 2006/014753 [pii]
AID - 10.1182/blood-2006-04-014753 [doi]
PST - ppublish
SO  - Blood. 2007 Apr 15;109(8):3173-6. doi: 10.1182/blood-2006-04-014753. Epub 2006 
      Dec 19.