PMID- 17179653 OWN - NLM STAT- MEDLINE DCOM- 20070618 LR - 20190608 IS - 0916-8818 (Print) IS - 0916-8818 (Linking) VI - 53 IP - 2 DP - 2007 Apr TI - Involvement of granulin in estrogen-induced neurogenesis in the adult rat hippocampus. PG - 297-307 AB - Recent studies have demonstrated the presence of neurogenesis in the adult mammalian hippocampus, and it has been suggested that estrogen and various growth factors influence the processes of adult neurogenesis. The present study assessed cell proliferation in the dentate gyrus and the mRNA expression levels of granulin, insulin-like growth factor-I (IGF-I), and brain-derived neurotrophic factor (BDNF) in the hippocampus 4 h after treatment with estradiol benzoate (EB) in 3- and 12-month old ovariectomized rats. At 3 months of age, mRNA expression of granulin precursor and cell proliferation were increased by EB treatment, although the mRNA expressions of IGF-I and BDNF remained unchanged. At 12 months of age, however, neither mRNA expression of the three genes nor cell proliferation in the dentate gyrus were affected by EB treatment. In addition, 17beta-estradiol enhanced the proliferation of neural progenitor cells derived from hippocampal tissue of 3-month-old female rats in vitro; this was inhibited by neutralization of granulin with specific antibody. These results suggest that estrogen induces granulin gene expression in the hippocampus and that the product of this gene is involved in the mitogenic effects of estrogen in the dentate gyrus, although the responses to estrogen decline with age. FAU - Chiba, Shuichi AU - Chiba S AD - Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo, Tokyo, Japan. FAU - Suzuki, Masatoshi AU - Suzuki M FAU - Yamanouchi, Keitaro AU - Yamanouchi K FAU - Nishihara, Masugi AU - Nishihara M LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20061220 PL - Japan TA - J Reprod Dev JT - The Journal of reproduction and development JID - 9438792 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Estrogens) RN - 0 (Intercellular Signaling Peptides and Proteins) RN - 0 (Progranulins) RN - 67763-96-6 (Insulin-Like Growth Factor I) SB - IM MH - Age Factors MH - Animals MH - Brain-Derived Neurotrophic Factor/drug effects/genetics MH - Cell Proliferation/drug effects MH - Cells, Cultured MH - Estrogens/*pharmacology MH - Female MH - Gene Expression Regulation/drug effects MH - Hippocampus/*cytology/drug effects/*physiology MH - Insulin-Like Growth Factor I/drug effects/genetics MH - Intercellular Signaling Peptides and Proteins/genetics/*metabolism MH - Neurons/drug effects/*metabolism MH - Ovariectomy MH - Progranulins MH - Rats MH - Rats, Wistar MH - Stem Cells/drug effects EDAT- 2006/12/21 09:00 MHDA- 2007/06/19 09:00 CRDT- 2006/12/21 09:00 PHST- 2006/12/21 09:00 [pubmed] PHST- 2007/06/19 09:00 [medline] PHST- 2006/12/21 09:00 [entrez] AID - JST.JSTAGE/jrd/18108 [pii] AID - 10.1262/jrd.18108 [doi] PST - ppublish SO - J Reprod Dev. 2007 Apr;53(2):297-307. doi: 10.1262/jrd.18108. Epub 2006 Dec 20.