PMID- 17180102
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20061220
IS  - 1350-9047 (Print)
IS  - 1350-9047 (Linking)
VI  - 3
IP  - 3
DP  - 1996 Jul
TI  - Imidazole antifungals Miconazole and Econazole induce apoptosis in mouse lymphoma 
      and human T cell leukemia cells: regulation by Bcl-2 and potential role of 
      calcium.
PG  - 331-7
AB  - We have recently reported that thapsigargin (TG), a specific endoplasmic 
      reticulum (ER)-associated Ca(2+)-ATPase inhibitor, induces apoptosis in mouse 
      lymphoma cells. In view of recent evidence that the imidazole antifungals 
      econazole (EC) and miconazole (MC) inhibit TG-sensitive Ca(2+)-ATPase activity in 
      normal rat thymocytes, we investigated the effect of these agents on 
      intracellular Ca(2+) homeostasis and cell survival in WEHI7.2 mouse lymphoma 
      cells and human CEMT-cell leukemia cells. In this report, we demonstrate that MC 
      treatment releases Ca(2+) from the TG-sensitive ER pool of WEHI7.2 cells. MC 
      induced apoptosis, based on morphological and biochemical criteria, and on 
      inhibition by the Bcl-2 oncogene. Moreover, intracellular Ca(2+) changes induced 
      by MC treatment were inhibited by overexpression of Bcl-2. In addition to 
      inducing cell death in WEHI7.2 cells, MC induced apoptosis in the glucocorticoid 
      sensitive and resistant human T-cell leukemia lines, CEM-C7 and CEM-C1 
      respectively, in normal thymocytes and in normal lymphocytes. Based on their 
      apoptosis-inducing activity, imidazole derivatives should be explored as 
      potential immunosuppressive and/or chemotherapeutic agents.
FAU - Sobecks, R
AU  - Sobecks R
AD  - Departments of Medicine and Pharmacology, Case Western Reserve University, 
      Cleveland, Ohio.
FAU - McCormick, T S
AU  - McCormick TS
FAU - Distelhorst, C W
AU  - Distelhorst CW
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cell Death Differ
JT  - Cell death and differentiation
JID - 9437445
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1995/12/12 00:00 [received]
PHST- 1996/02/22 00:00 [revised]
PHST- 1996/03/11 00:00 [accepted]
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
PST - ppublish
SO  - Cell Death Differ. 1996 Jul;3(3):331-7.