PMID- 17180681
OWN - NLM
STAT- MEDLINE
DCOM- 20070327
LR  - 20201226
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 59
IP  - 2
DP  - 2007 Feb
TI  - Patterns of constitutive and IFN-gamma inducible expression of HLA class II 
      molecules in human melanoma cell lines.
PG  - 123-33
AB  - Major histocompatibility complex (MHC) class II proteins (HLA-DR, HLA-DP and 
      HLA-DQ) play a fundamental role in the regulation of the immune response. The 
      level of expression of human leukocyte antigen (HLA) class II antigens is 
      regulated by interferon-gamma (IFN-gamma) and depends on the status of class II 
      trans-activator protein (CIITA), a co-activator of the MHC class II gene 
      promoter. In this study, we measured levels of constitutive and IFN-gamma-induced 
      expression of MHC class II molecules, analysed the expression of CIITA and 
      investigated the association between MHC class II transactivator polymorphism and 
      expression of different MHC class II molecules in a large panel of melanoma cell 
      lines obtained from the European Searchable Tumour Cell Line Database. Many cell 
      lines showed no constitutive expression of HLA-DP, HLA-DQ and HLA-DR and no 
      IFN-gamma-induced increase in HLA class II surface expression. However, in some 
      cases, IFN-gamma treatment led to enhanced surface expression of HLA-DP and 
      HLA-DR. HLA-DQ was less frequently expressed under basal conditions and was less 
      frequently induced by IFN-gamma. In these melanoma cell lines, constitutive 
      surface expression of HLA-DR and HLA-DP was higher than that of HLA-DQ. In 
      addition, high constitutive level of cell surface expression of HLA-DR was 
      correlated with lower inducibility of this expression by IFN-gamma. Finally, 
      substitution A-->G in the 5' flanking region of CIITA promoter type III was 
      associated with higher expression of constitutive HLA-DR (p<0.005). This study 
      yielded a panel of melanoma cell lines with different patterns of constitutive 
      and IFN-gamma-induced expression of HLA class II that can be used in future 
      studies of the mechanisms of regulation of HLA class II expression.
FAU - Rodriguez, T
AU  - Rodriguez T
AD  - Departamento de Analisis Clinicos, Hospital Universitario Virgen de las Nieves, 
      Universidad de Granada, Avda. Fuerzas Armadas 2, 18014, Granada, Spain.
FAU - Mendez, R
AU  - Mendez R
FAU - Del Campo, A
AU  - Del Campo A
FAU - Aptsiauri, N
AU  - Aptsiauri N
FAU - Martin, J
AU  - Martin J
FAU - Orozco, G
AU  - Orozco G
FAU - Pawelec, G
AU  - Pawelec G
FAU - Schadendorf, D
AU  - Schadendorf D
FAU - Ruiz-Cabello, F
AU  - Ruiz-Cabello F
FAU - Garrido, F
AU  - Garrido F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061220
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (DNA Probes)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (MHC class II transactivator protein)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Trans-Activators)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Alleles
MH  - Base Sequence
MH  - Cell Line, Tumor
MH  - DNA Probes/genetics
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - *Genes, MHC Class II
MH  - HLA-DR Antigens/genetics
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Melanoma/*genetics/*immunology/metabolism
MH  - Nuclear Proteins/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Neoplasm/genetics/metabolism
MH  - Recombinant Proteins
MH  - Trans-Activators/genetics
EDAT- 2006/12/21 09:00
MHDA- 2007/03/28 09:00
CRDT- 2006/12/21 09:00
PHST- 2006/07/21 00:00 [received]
PHST- 2006/10/19 00:00 [accepted]
PHST- 2006/12/21 09:00 [pubmed]
PHST- 2007/03/28 09:00 [medline]
PHST- 2006/12/21 09:00 [entrez]
AID - 10.1007/s00251-006-0171-9 [doi]
PST - ppublish
SO  - Immunogenetics. 2007 Feb;59(2):123-33. doi: 10.1007/s00251-006-0171-9. Epub 2006 
      Dec 20.