PMID- 17180710
OWN - NLM
STAT- MEDLINE
DCOM- 20070627
LR  - 20131121
IS  - 1387-2176 (Print)
IS  - 1387-2176 (Linking)
VI  - 9
IP  - 2
DP  - 2007 Apr
TI  - Manufacturing monodisperse chitosan microparticles containing ampicillin using a 
      microchannel chip.
PG  - 253-9
AB  - The purpose of this study was using a developed microfluidic chip to prepare 
      size-controlled monodisperse chitosan microparticles encapsulating ampicillin. 
      Our strategy is that a chitosan aqueous solution (the disperse phase) is fed into 
      the microfluidic chip equipped with a cross-junction microchannel, and is sheared 
      by the viscous oil flows (the continuous phase) to form monodisperse 
      semi-product, chitosan emulsions. These fine emulsions are then gelled into 
      stability upon gelation by injection of copper sulfate solution at the terminal 
      microchannel of the microfluidic chip, and finally the uniform chitosan 
      microparticles are formed in an efficient manner. The proposed chip is fabricated 
      by a CO(2) laser machine on a conventional poly methyl methacrylate (PMMA) 
      substrate. This microfluidic chip has four inlet ports, one cross-channel and one 
      outlet port. We have demonstrated that one can control the size of chitosan 
      microparticles from 100 to 800 microm in diameter (with a variation less than 5%) 
      by altering the relative sheath/sample flow rate ratio. Experimental data showed 
      that when given a steady continuous phase (oil flow), the emulsion size increases 
      with the increase in average velocity of the dispersed phase flow (sample flow). 
      In addition, the release of the model drug (ampicillin) from these microspheres 
      is proved to be once-daily for clinical application. We also revealed that 
      appropriate particle sizes for different release patterns are predictable, 
      enabling better applications of chitosan as a drug carrier.
FAU - Yang, Chih-Hui
AU  - Yang CH
AD  - Department of Biological Science and Technology, I-Shou University, Kaohsiung, 
      Taiwan, R.O.C. chyang@isu.edu.tw
FAU - Huang, Keng-Shiang
AU  - Huang KS
FAU - Chang, Jia-Yaw
AU  - Chang JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biomed Microdevices
JT  - Biomedical microdevices
JID - 100887374
RN  - 0 (Capsules)
RN  - 0 (Drug Carriers)
RN  - 7C782967RD (Ampicillin)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Ampicillin/*administration & dosage/*chemistry
MH  - Capsules/chemistry
MH  - Chitosan/*chemical synthesis
MH  - Drug Carriers/*chemistry
MH  - Drug Compounding/*methods
MH  - Materials Testing
MH  - Microfluidic Analytical Techniques/*methods
MH  - Particle Size
EDAT- 2006/12/21 09:00
MHDA- 2007/06/28 09:00
CRDT- 2006/12/21 09:00
PHST- 2006/12/21 09:00 [pubmed]
PHST- 2007/06/28 09:00 [medline]
PHST- 2006/12/21 09:00 [entrez]
AID - 10.1007/s10544-006-9029-z [doi]
PST - ppublish
SO  - Biomed Microdevices. 2007 Apr;9(2):253-9. doi: 10.1007/s10544-006-9029-z.