PMID- 17184987
OWN - NLM
STAT- MEDLINE
DCOM- 20070409
LR  - 20131121
IS  - 0959-8049 (Print)
IS  - 0959-8049 (Linking)
VI  - 43
IP  - 2
DP  - 2007 Jan
TI  - Reconstituted expression of menin in Men1-deficient mouse Leydig tumour cells 
      induces cell cycle arrest and apoptosis.
PG  - 402-14
AB  - Multiple endocrine neoplasia type 1 (MEN1) is a hereditary syndrome caused by the 
      inactivation of the responsible gene, MEN1. To date, the lack of MEN1-deficient 
      cell lines derived directly from MEN1 tumours has hampered the detailed study of 
      the MEN1 gene. We have established several stable Men1-deficient Leydig cell 
      tumour (LCT) lines derived from a Leydig cell tumour developed in a male 
      heterozygous Men1 mutant mouse. Our data show that these cell lines maintain the 
      basic characteristics of Leydig cells in terms of both androgen synthesis and 
      gene expression. Interestingly, reconstituted menin expression in one of 
      Men1-deficient LCT cell lines resulted in cell growth inhibition, suggesting that 
      the function of cell growth suppression of the menin pathway, apart from menin 
      itself, is essentially preserved in these cells. Furthermore, we show that menin 
      re-expression in these Men1-deficient cells leads to a block in the transition 
      from G0/G1 to S phase of the cell cycle and an increase in apoptosis, accompanied 
      by a marked increase of p18INK4C and p27Kip1 expression. The current study 
      therefore highlights the importance of menin expression in cell cycle and cell 
      survival control in endocrine cells, and may provide insights into the mechanisms 
      of tumour suppression by menin in related endocrine tumours.
FAU - Hussein, Nader
AU  - Hussein N
AD  - Laboratoire Genetique Moleculaire, Signalisation et Cancer, CNRS, UMR5201, 
      Faculte de Medecine, Universite Claude Bernard Lyon 1, 8 Ave. Rockefeller, 69373 
      Lyon, France.
FAU - Casse, Huguette
AU  - Casse H
FAU - Fontaniere, Sandra
AU  - Fontaniere S
FAU - Morera, Anne-Marie
AU  - Morera AM
FAU - Asensio, Marie J
AU  - Asensio MJ
FAU - Bakeli, Skander
AU  - Bakeli S
FAU - Lu, Jie L
AU  - Lu JL
FAU - Coste, Isabelle
AU  - Coste I
FAU - Di Clemente, Nathalie
AU  - Di Clemente N
FAU - Bertolino, Philippe
AU  - Bertolino P
FAU - Zhang, Chang X
AU  - Zhang CX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061220
PL  - England
TA  - Eur J Cancer
JT  - European journal of cancer (Oxford, England : 1990)
JID - 9005373
RN  - 0 (MEN1 protein, human)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Cell Cycle/*genetics
MH  - Cell Line, Tumor
MH  - Immunohistochemistry
MH  - Leydig Cell Tumor/*genetics/metabolism
MH  - Loss of Heterozygosity/genetics
MH  - Male
MH  - Mice
MH  - Multiple Endocrine Neoplasia Type 1/*genetics/metabolism
MH  - Mutation/*genetics
MH  - Progesterone/metabolism
MH  - Proto-Oncogene Proteins/deficiency/*genetics/metabolism
EDAT- 2006/12/23 09:00
MHDA- 2007/04/10 09:00
CRDT- 2006/12/23 09:00
PHST- 2006/06/26 00:00 [received]
PHST- 2006/08/28 00:00 [revised]
PHST- 2006/08/31 00:00 [accepted]
PHST- 2006/12/23 09:00 [pubmed]
PHST- 2007/04/10 09:00 [medline]
PHST- 2006/12/23 09:00 [entrez]
AID - S0959-8049(06)00961-0 [pii]
AID - 10.1016/j.ejca.2006.08.038 [doi]
PST - ppublish
SO  - Eur J Cancer. 2007 Jan;43(2):402-14. doi: 10.1016/j.ejca.2006.08.038. Epub 2006 
      Dec 20.