PMID- 1718543
OWN - NLM
STAT- MEDLINE
DCOM- 19911213
LR  - 20190613
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 553
IP  - 2
DP  - 1991 Jul 12
TI  - Role of voltage-sensitive calcium channels in mitogenic stimulation of 
      neuroblasts.
PG  - 211-4
AB  - The present study examines the role of Ca2+ in the regulation of sympathetic 
      neuroblast mitosis. Employing a fully defined neuroblast culture system, we 
      previously found that insulin growth factors (IGFs), depolarization and 
      vasoactive intestinal peptide (VIP) regulated precursor mitosis. We now report 
      that Ca2+ entry via voltage-sensitive channels was required for 
      depolarization-stimulated mitogenesis. Ca2+ channel blockade with nitrendipine 
      completely inhibited the increase in [3H]thymidine incorporation elicited by 
      depolarizing stimuli including 30 mM KCl and the Na+ channel agonist veratridine. 
      However, Ca2+ channel activity was not involved in the stimulation of DNA 
      synthesis by IGFs or VIP. Thus, neuroblast mitosis may be regulated by multiple 
      intracellular as well as extracellular signals.
FAU - Pincus, D W
AU  - Pincus DW
AD  - Department of Neurology, Cornell University Medical College, New York, NY 10021.
FAU - DiCicco-Bloom, E
AU  - DiCicco-Bloom E
FAU - Black, I B
AU  - Black IB
LA  - eng
GR  - HD23315/HD/NICHD NIH HHS/United States
GR  - NS10259/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Calcium Channels)
RN  - 0 (Insulin)
RN  - 37221-79-7 (Vasoactive Intestinal Peptide)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 660YQ98I10 (Potassium Chloride)
RN  - 71-62-5 (Veratridine)
RN  - 71145-03-4 (3-Pyridinecarboxylic acid, 
      1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester)
RN  - 9B627AW319 (Nitrendipine)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - 3-Pyridinecarboxylic acid, 
      1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl 
      ester/pharmacology
MH  - Analysis of Variance
MH  - Animals
MH  - Calcium Channel Blockers/*pharmacology
MH  - Calcium Channels/drug effects/*physiology
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - DNA Replication/drug effects
MH  - Ganglia, Sympathetic/cytology/drug effects/*physiology
MH  - Insulin/pharmacology
MH  - Male
MH  - Models, Neurological
MH  - Neurons/cytology/drug effects/*physiology
MH  - Nitrendipine/pharmacology
MH  - Potassium Chloride/pharmacology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Tetrodotoxin/*pharmacology
MH  - Thymidine/metabolism
MH  - Vasoactive Intestinal Peptide/pharmacology
MH  - Veratridine/pharmacology
EDAT- 1991/07/12 00:00
MHDA- 1991/07/12 00:01
CRDT- 1991/07/12 00:00
PHST- 1991/07/12 00:00 [pubmed]
PHST- 1991/07/12 00:01 [medline]
PHST- 1991/07/12 00:00 [entrez]
AID - 0006-8993(91)90827-I [pii]
AID - 10.1016/0006-8993(91)90827-i [doi]
PST - ppublish
SO  - Brain Res. 1991 Jul 12;553(2):211-4. doi: 10.1016/0006-8993(91)90827-i.