PMID- 17185762
OWN - NLM
STAT- MEDLINE
DCOM- 20070629
LR  - 20071203
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 373
DP  - 2007
TI  - Pyrosequencing-based strategies for improved allele typing of human leukocyte 
      antigen loci.
PG  - 115-34
AB  - Successful transplantation of tissue during solid organ and bone marrow 
      transplantation relies on accurate determination of the human leukocyte antigen 
      (HLA) phenotype of the potential donor(s) and recipient. Matching donor with 
      recipient for a kidney transplant generally means finding a six-antigen match by 
      looking at each of two alleles at HLA-A, -B, and -DR loci. For bone marrow 
      transplantation the HLA-C and -DQ alleles are also considered. Molecular 
      techniques, including sequencing, are capable of precisely defining HLA alleles. 
      Because of the large number of possible allelic combinations there are numerous 
      ambiguities associated with heterozygous genotypes even when sequence-based 
      typing protocols are used. Sequencing-by-synthesis methodology employed by 
      Pyrosequencing represents an improvement when applied to HLA genotyping that 
      allows resolution of many ambiguous allelic pairs. Out-of-phase sequencing of HLA 
      alleles by Pyrosequencing can resolve cis/trans ambiguities that would otherwise 
      require the sequencing of isolated cloned DNAs. Single-nucleotide polymorphism 
      typing of HLA for the presence of specific variants is also beneficial for 
      monitoring HLA-encoded genetic risk to autoimmune diseases, such as celiac 
      disease, rheumatoid arthritis, and type 1 diabetes mellitus.
FAU - Ringquist, Steven
AU  - Ringquist S
AD  - Division of Immunogenetics, Department of Pediatrics, Rangos Research Center, 
      Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, 
      Pittsburgh, PA, USA.
FAU - Styche, Alexis
AU  - Styche A
FAU - Rudert, William A
AU  - Rudert WA
FAU - Trucco, Massimo
AU  - Trucco M
LA  - eng
GR  - R01 DK 2402/DK/NIDDK NIH HHS/United States
GR  - U19 AI 056374-01/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Diphosphates)
RN  - 0 (HLA Antigens)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Alleles
MH  - Base Sequence
MH  - Chromosomes, Human, Pair 6/genetics
MH  - DNA/blood/isolation & purification
MH  - Diphosphates/*metabolism
MH  - Electrophoresis, Agar Gel
MH  - Genotype
MH  - HLA Antigens/classification/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis, DNA/*methods
MH  - Specimen Handling
EDAT- 2006/12/23 09:00
MHDA- 2007/06/30 09:00
CRDT- 2006/12/23 09:00
PHST- 2006/12/23 09:00 [pubmed]
PHST- 2007/06/30 09:00 [medline]
PHST- 2006/12/23 09:00 [entrez]
AID - 1-59745-377-3:115 [pii]
AID - 10.1385/1-59745-377-3:115 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2007;373:115-34. doi: 10.1385/1-59745-377-3:115.