PMID- 17186142
OWN - NLM
STAT- MEDLINE
DCOM- 20070924
LR  - 20181113
IS  - 0095-3628 (Print)
IS  - 0095-3628 (Linking)
VI  - 54
IP  - 1
DP  - 2007 Jul
TI  - Molecular monitoring of microbial population dynamics during operational periods 
      of anaerobic hybrid reactor treating cassava starch wastewater.
PG  - 21-30
AB  - This study characterized the microbial community and population dynamics in an 
      anaerobic hybrid reactor (AHR) treating cassava starch wastewater. Methanogens 
      and nonmethanogens were followed during the start-up and operation of the 
      reactor, and linked to operational and performance data. Biomass samples taken 
      from the sludge bed and packed bed zones of the AHR at intervals throughout the 
      operational period were examined by 16S rRNA fluorescence in situ hybridization 
      (FISH). The start-up seed and the reactor biomass were sampled during the feeding 
      of the wastewater with a chemical oxygen demand (COD) value of 8 g L(-1) and a 
      hydraulic retention time (HRT) of 8 days. These samples were characterized by the 
      predominance of cells with long-rod morphology similar to Methanosaeta spp. 
      Following a sharp operational change, accomplished by increasing the COD 
      concentration of the organic influent from 8 to 10 g L(-1) and reducing the HRT 
      from 8 to 5 days, there was a doubling of the organic loading rate, a reduction 
      of the COD removal efficiency, as well as decreased methane content in the biogas 
      and an accumulation of total volatile acids in the reactor. Moreover, this 
      operational change resulted in a significant population shift from long-rod 
      Methanosaeta-like cells to tetrad-forming Methanosarcina-like cells. The 
      distributions of microbial populations involved in different zones of the AHR 
      were determined. The results showed that nonmethanogens became the predominant 
      population in both sludge and the packed bed zone. However, the percentage of 
      methanogens in the packed bed zone was higher than that in the sludge bed zone. 
      This higher percentage of methanogens was likely caused by the fact that the 
      packed bed zone provided a suitable environmental condition with an appropriate 
      nutrient availability for methanogen growth.
FAU - Boonapatcharoen, Nimaradee
AU  - Boonapatcharoen N
AD  - School of Bioresources and Technology, King Mongkut University of Technology 
      Thonburi, Bangkok, Thailand.
FAU - Meepian, Kulyanee
AU  - Meepian K
FAU - Chaiprasert, Pawinee
AU  - Chaiprasert P
FAU - Techkarnjanaruk, Somkiet
AU  - Techkarnjanaruk S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061222
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 059QF0KO0R (Water)
RN  - 9005-25-8 (Starch)
SB  - IM
MH  - Bacteria, Anaerobic/classification/genetics/*metabolism
MH  - Biodegradation, Environmental
MH  - Bioreactors/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - *Manihot
MH  - Starch/*metabolism
MH  - Water/chemistry
MH  - *Water Purification
EDAT- 2006/12/23 09:00
MHDA- 2007/09/25 09:00
CRDT- 2006/12/23 09:00
PHST- 2006/08/15 00:00 [received]
PHST- 2006/09/07 00:00 [accepted]
PHST- 2006/08/15 00:00 [revised]
PHST- 2006/12/23 09:00 [pubmed]
PHST- 2007/09/25 09:00 [medline]
PHST- 2006/12/23 09:00 [entrez]
AID - 10.1007/s00248-006-9161-6 [doi]
PST - ppublish
SO  - Microb Ecol. 2007 Jul;54(1):21-30. doi: 10.1007/s00248-006-9161-6. Epub 2006 Dec 
      22.