PMID- 17186341
OWN - NLM
STAT- MEDLINE
DCOM- 20071016
LR  - 20181113
IS  - 0941-1291 (Print)
IS  - 0941-1291 (Linking)
VI  - 37
IP  - 1
DP  - 2007
TI  - Efficacy and limitations of natural killer cell depletion in 
      cyclophosphamide-induced tolerance.
PG  - 24-9
AB  - PURPOSE: We previously developed a cyclophosphamide (CP)-induced tolerance 
      protocol, consisting of an intravenous injection of 1 x 10(8) donor spleen cells 
      (SC) given on day 0 and an intraperitoneal injection of 200 mg/kg CP given on day 
      2. In the present study, we modified this protocol with natural killer cell (NK) 
      depletion in recipient mice, and evaluated the efficacy of tolerance induction. 
      METHODS: We used B10.D2 (H-2d; IE+) and B10 (H-2b; IE-) mice as both donors and 
      recipients. The recipient mice were treated with donor SC, CP, and donor bone 
      marrow cells (BMCs) with or without NK depletion. RESULTS: A higher level of 
      mixed chimerism was achieved in the NK-depleted recipients. Survival of both the 
      skin and heart donor grafts was significantly prolonged in the NK-depleted 
      recipients. Donor reactive Vbeta11+ T cells were found at the same level as in 
      untreated control mice. Pretreatment with recipient NK cell depletion was 
      effective in inducing higher levels of donor mixed chimerism; however, permanent 
      engraftment of donor bone marrow was not achieved. CONCLUSION: Survival of donor 
      grafts was remarkably prolonged in the NK cell-depleted group, but 
      transplantation tolerance could not be induced. Our results suggest that NK cell 
      depletion in CP-induced tolerance conditioning has some effect on the induction 
      of donor-specific tolerance.
FAU - Shimizu, Ichiro
AU  - Shimizu I
AD  - Department of Cardiovascular Surgery, Kyushu University, 3-1-1 Maidashi, Fukuoka 
      812-8582, Japan.
FAU - Tomita, Yukihiro
AU  - Tomita Y
FAU - Okano, Shinji
AU  - Okano S
FAU - Iwai, Toshiro
AU  - Iwai T
FAU - Kajiwara, Takashi
AU  - Kajiwara T
FAU - Onzuka, Tatsushi
AU  - Onzuka T
FAU - Tominaga, Ryuji
AU  - Tominaga R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070101
PL  - Japan
TA  - Surg Today
JT  - Surgery today
JID - 9204360
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Surface)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Lectins, C-Type)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily B)
RN  - 8N3DW7272P (Cyclophosphamide)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antigens, Surface/immunology
MH  - *Chimerism
MH  - Cyclophosphamide/*immunology
MH  - Heart Transplantation/immunology
MH  - Immune Tolerance/*immunology
MH  - Immunosuppressive Agents/*immunology
MH  - Killer Cells, Natural/*immunology
MH  - Lectins, C-Type/immunology
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Models, Animal
MH  - NK Cell Lectin-Like Receptor Subfamily B
MH  - Skin Transplantation/immunology
MH  - Transplantation Immunology
MH  - Treatment Outcome
EDAT- 2006/12/23 09:00
MHDA- 2007/10/17 09:00
CRDT- 2006/12/23 09:00
PHST- 2006/04/24 00:00 [received]
PHST- 2006/06/04 00:00 [accepted]
PHST- 2006/12/23 09:00 [pubmed]
PHST- 2007/10/17 09:00 [medline]
PHST- 2006/12/23 09:00 [entrez]
AID - 10.1007/s00595-006-3329-z [doi]
PST - ppublish
SO  - Surg Today. 2007;37(1):24-9. doi: 10.1007/s00595-006-3329-z. Epub 2007 Jan 1.