PMID- 17187385
OWN - NLM
STAT- MEDLINE
DCOM- 20070625
LR  - 20191210
IS  - 1549-3296 (Print)
IS  - 1549-3296 (Linking)
VI  - 81
IP  - 3
DP  - 2007 Jun 1
TI  - Evaluation of the biological responses of osteoblast-like UMR-106 cells to the 
      engineered porous PHBV matrix.
PG  - 669-77
AB  - Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) has been investigated for 
      biomedical applications due to its many biologically favorable properties. 
      However, to explore its application in bone tissue engineering, the poorly 
      bioactive surface property of PHBV must be improved. To engineer PHBV to achieve 
      a biologically active surface, in this study each porous PHBV matrix was prepared 
      by solute leaching of salt/PHBV cast film and was treated with ozone followed by 
      dip coating with type I collagen. The biological responses of osteoblast-like 
      UMR-106 cells after being grown on the engineered PHBV matrix were evaluated. 
      Confocal microscopy and the MTT assay were used to map and quantify the viable 
      cell proliferation on the PHBV matrix, respectively. The cells were cultivated in 
      osteogenic media containing beta-glycerophosphate and later stained with alizarin 
      red to visualize mineralization of the matrix. RNA was extracted from the UMR-106 
      cells, and reverse transcriptase-polymerase chain reaction (RT-PCR) was applied 
      to detect expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (a house 
      keeping gene) and bone sialoprotein (BSP) (marker of the osteoblastic phenotype). 
      The results showed that the UMR-106 cells after cultivation on the engineered 
      PHBV matrix retained the osteoblastic phenotype characteristics, indicating that 
      the porous PHBV matrix after ozone treatment and collagen dip coatings are a 
      promising scaffold for bone tissue engineering applications.
FAU - Liu, Hui
AU  - Liu H
AD  - Polymer Group, Department of Chemistry, Howard University, Washington District of 
      Columbia 20059, USA.
FAU - Raghavan, Dharmaraj
AU  - Raghavan D
FAU - Stubbs, John 3rd
AU  - Stubbs J 3rd
LA  - eng
GR  - S06GM 008016-35/GM/NIGMS NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Anthraquinones)
RN  - 0 (Formazans)
RN  - 0 (Integrin-Binding Sialoprotein)
RN  - 0 (Polyesters)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sialoglycoproteins)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate))
RN  - 23305-68-2 (MTT formazan)
RN  - 60MEW57T9G (alizarin)
SB  - IM
MH  - Animals
MH  - Anthraquinones
MH  - Calcification, Physiologic/drug effects
MH  - Cattle
MH  - Cell Proliferation/drug effects
MH  - Formazans
MH  - Gene Expression Regulation/drug effects
MH  - Integrin-Binding Sialoprotein
MH  - Microscopy, Confocal
MH  - Osteoblasts/cytology/*drug effects/*metabolism
MH  - Polyesters/*pharmacology
MH  - Porosity/drug effects
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Sialoglycoproteins/genetics/metabolism
MH  - Tetrazolium Salts
MH  - Tissue Engineering/*methods
EDAT- 2006/12/26 09:00
MHDA- 2007/06/26 09:00
CRDT- 2006/12/26 09:00
PHST- 2006/12/26 09:00 [pubmed]
PHST- 2007/06/26 09:00 [medline]
PHST- 2006/12/26 09:00 [entrez]
AID - 10.1002/jbm.a.31101 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2007 Jun 1;81(3):669-77. doi: 10.1002/jbm.a.31101.