PMID- 17188139
OWN - NLM
STAT- MEDLINE
DCOM- 20070130
LR  - 20070716
IS  - 0039-6060 (Print)
IS  - 0039-6060 (Linking)
VI  - 140
IP  - 6
DP  - 2006 Dec
TI  - Recognizing genes differentially regulated in vitro by the multiple endocrine 
      neoplasia type 1 (MEN1) gene, using RNA interference and oligonucleotide 
      microarrays.
PG  - 921-9; discussion 929-31
AB  - BACKGROUND: Data on downstream effects of MEN1 gene inactivation is scarce. In an 
      effort to identify genes regulated by MEN1, we designed a silencing experiment in 
      a human endocrine pancreatic tumor cell line (BON1). METHODS: By using RNA 
      interference, MEN1 mRNA expression was knocked-down by >85%. Gene expression was 
      assessed by oligonucleotide microarrays and compared to expression in nonsilenced 
      controls. We also investigated if genes were differentially expressed in 6 
      malignant endocrine pancreatic tumors (EPTs) with homozygous MEN1 inactivation 
      compared to 2 without MEN1 gene alterations. RESULTS: Using a cut-off of > or =2 
      times, 66 genes were found to be upregulated, and 22 were downregulated in the 
      MEN1-silenced clones. We corroborated the microarray findings by performing 
      quantitative-PCR on the RNA from the silencing experiments for 7 of the 88 
      differentially regulated genes. Genes involved in endocrine cell fate 
      determination, as well as genes known to be involved in NFkappaB, Notch, and Wnt 
      signaling pathways, were among genes verified as differentially regulated in 
      vitro. CONCLUSIONS: The demonstration of pathways affected by silencing of MEN1 
      in vitro provides novel insight into neoplastic processes of potential importance 
      in vivo, which warrants further study.
FAU - Stalberg, Peter
AU  - Stalberg P
AD  - Department of Surgical Sciences, University Hospital, Uppsala, Sweden.
FAU - Santesson, Marten
AU  - Santesson M
FAU - Ekeblad, Sara
AU  - Ekeblad S
FAU - Lejonklou, Margareta Halin
AU  - Lejonklou MH
FAU - Skogseid, Britt
AU  - Skogseid B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061101
PL  - United States
TA  - Surgery
JT  - Surgery
JID - 0417347
RN  - 0 (DNA, Neoplasm)
RN  - 0 (MEN1 protein, human)
RN  - 0 (NF-kappa B)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Receptors, Notch)
RN  - 0 (Wnt Proteins)
SB  - IM
CIN - Surgery. 2007 Jun;141(6):830-1. PMID: 17560264
MH  - Cell Line, Tumor
MH  - DNA, Neoplasm/genetics
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic/*physiology
MH  - Genes, Neoplasm/*genetics/physiology
MH  - Humans
MH  - NF-kappa B/genetics/metabolism
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Pancreatic Neoplasms/genetics/metabolism/pathology
MH  - Proto-Oncogene Proteins/*genetics/metabolism
MH  - *RNA Interference
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Neoplasm/genetics
MH  - Receptors, Notch/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transfection
MH  - Wnt Proteins/genetics/metabolism
EDAT- 2006/12/26 09:00
MHDA- 2007/01/31 09:00
CRDT- 2006/12/26 09:00
PHST- 2006/03/25 00:00 [received]
PHST- 2006/06/30 00:00 [accepted]
PHST- 2006/12/26 09:00 [pubmed]
PHST- 2007/01/31 09:00 [medline]
PHST- 2006/12/26 09:00 [entrez]
AID - S0039-6060(06)00494-6 [pii]
AID - 10.1016/j.surg.2006.06.038 [doi]
PST - ppublish
SO  - Surgery. 2006 Dec;140(6):921-9; discussion 929-31. doi: 
      10.1016/j.surg.2006.06.038. Epub 2006 Nov 1.