PMID- 17188747
OWN - NLM
STAT- MEDLINE
DCOM- 20070404
LR  - 20220310
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 28
IP  - 9
DP  - 2007 Mar
TI  - Biocompatibility evaluation of silk fibroin with peripheral nerve tissues and 
      cells in vitro.
PG  - 1643-52
AB  - Silk-based materials have been used in the field of bone or ligament tissue 
      engineering. In order to explore the feasibility of using purified silk fibroin 
      to construct artificial nerve grafts, it is necessary to evaluate the 
      biocompatibility of silk fibroin material with peripheral nerve tissues and 
      cells. We cultured rat dorsal root ganglia (DRG) on the substrate made up of silk 
      fibroin fibers and observed the cell outgrowth from DRG during culture by using 
      light and electron microscopy coupled with immunocytochemistry. On the other 
      hand, we cultured Schwann cells from rat sciatic nerves in the silk fibroin 
      extract fluid and examined the changes of Schwann cells after different times of 
      culture. The results of light microscopy, MTT test and cell cycle analysis showed 
      that Schwann cells cultured in the silk fibroin extract fluid showed no 
      significant difference in their morphology, cell viability and proliferation as 
      compared to that in plain L15 medium. Furthermore, no significant difference was 
      found in expression of the factors secreted by Schwann cells, such as nerve 
      growth factor (NGF), brain-derived neurotrophic factor (BDNF) and S-100, between 
      Schwann cells cultured in the silk fibroin extraction fluid and in plain L15 
      medium by the aid of immunocytochemistry, RT-PCR and Western analysis. 
      Collectively, these data indicate that silk fibroin has good biocompatibility 
      with DRG and is also beneficial to the survival of Schwann cells without exerting 
      any significant cytotoxic effects on their phenotype or functions, thus providing 
      an experimental foundation for the development of silk fibroin as a candidate 
      material for nerve tissue engineering applications.
FAU - Yang, Yumin
AU  - Yang Y
AD  - Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 19 
      Qixiu Road, Jiangsu Province 226001, PR China.
FAU - Chen, Xuemei
AU  - Chen X
FAU - Ding, Fei
AU  - Ding F
FAU - Zhang, Peiyun
AU  - Zhang P
FAU - Liu, Jie
AU  - Liu J
FAU - Gu, Xiaosong
AU  - Gu X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061226
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Biocompatible Materials)
RN  - 9007-76-5 (Fibroins)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Apoptosis/drug effects
MH  - Biocompatible Materials/chemistry/*pharmacology
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Drug Evaluation, Preclinical
MH  - Fibroins/*pharmacology
MH  - Ganglia, Spinal/*cytology/*drug effects
MH  - In Vitro Techniques
MH  - Materials Testing
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Schwann Cells/*cytology/*drug effects
EDAT- 2006/12/26 09:00
MHDA- 2007/04/05 09:00
CRDT- 2006/12/26 09:00
PHST- 2006/09/01 00:00 [received]
PHST- 2006/12/01 00:00 [accepted]
PHST- 2006/12/26 09:00 [pubmed]
PHST- 2007/04/05 09:00 [medline]
PHST- 2006/12/26 09:00 [entrez]
AID - S0142-9612(06)01019-2 [pii]
AID - 10.1016/j.biomaterials.2006.12.004 [doi]
PST - ppublish
SO  - Biomaterials. 2007 Mar;28(9):1643-52. doi: 10.1016/j.biomaterials.2006.12.004. 
      Epub 2006 Dec 26.