PMID- 17188757
OWN - NLM
STAT- MEDLINE
DCOM- 20070425
LR  - 20070226
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 141
IP  - 1
DP  - 2007 Apr
TI  - Simple and rapid detection of the porcine reproductive and respiratory syndrome 
      virus from pig whole blood using filter paper.
PG  - 102-6
AB  - The combination of Flinders Technology Associates filter papers (FTA cards) and 
      real-time PCR was examined to establish a simple and rapid technique for the 
      detection of porcine reproductive and respiratory syndrome virus (PRRSV) from 
      whole pig blood. A modified live PRRS vaccine was diluted with either sterilised 
      saline or pig whole blood, and the suspensions were applied onto the FTA cards. 
      The real-time RT-PCR detection of PRRSV was performed directly with the samples 
      applied to the FTA card without the RNA extraction step. Six whole blood samples 
      from at random selected piglets in the PRRSV infected farm were also assayed in 
      this study. The expected PCR product was successfully amplified from either 
      saline diluted or pig whole blood diluted vaccine. The same PCR ampliocon was 
      detected from all blood samples assayed in this study. This study suggested that 
      the combination of an FTA card and real-time PCR is a rapid and easy technique 
      for the detection of PRRSV. This technique can remarkably shorten the time 
      required for PRRSV detection from whole blood and makes the procedure much 
      easier.
FAU - Inoue, Ryo
AU  - Inoue R
AD  - Kyoto Prefectural University, Laboratory of Animal Science, 1-5 Shimogamo, Kyoto 
      606-8522, Japan.
FAU - Tsukahara, Takamitsu
AU  - Tsukahara T
FAU - Sunaba, Chinatsu
AU  - Sunaba C
FAU - Itoh, Mitsugi
AU  - Itoh M
FAU - Ushida, Kazunari
AU  - Ushida K
LA  - eng
PT  - Journal Article
DEP - 20061226
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Filtration/veterinary
MH  - Molecular Sequence Data
MH  - Nucleic Acid Amplification Techniques/veterinary
MH  - Paper
MH  - Polymerase Chain Reaction/*veterinary
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - RNA, Viral/*analysis
MH  - Sequence Homology, Nucleic Acid
MH  - Sus scrofa/*blood/*virology
MH  - Time Factors
EDAT- 2006/12/26 09:00
MHDA- 2007/04/26 09:00
CRDT- 2006/12/26 09:00
PHST- 2006/07/25 00:00 [received]
PHST- 2006/11/17 00:00 [revised]
PHST- 2006/11/23 00:00 [accepted]
PHST- 2006/12/26 09:00 [pubmed]
PHST- 2007/04/26 09:00 [medline]
PHST- 2006/12/26 09:00 [entrez]
AID - S0166-0934(06)00416-2 [pii]
AID - 10.1016/j.jviromet.2006.11.030 [doi]
PST - ppublish
SO  - J Virol Methods. 2007 Apr;141(1):102-6. doi: 10.1016/j.jviromet.2006.11.030. Epub 
      2006 Dec 26.