PMID- 17189315
OWN - NLM
STAT- MEDLINE
DCOM- 20070516
LR  - 20231213
IS  - 0006-3495 (Print)
IS  - 1542-0086 (Electronic)
IS  - 0006-3495 (Linking)
VI  - 92
IP  - 6
DP  - 2007 Mar 15
TI  - Gating properties of heterotypic gap junction channels formed of connexins 40, 
      43, and 45.
PG  - 1952-65
AB  - Connexins (Cxs) 40, 43, and 45 are expressed in many different tissues, but most 
      abundantly in the heart, blood vessels, and the nervous system. We examined 
      formation and gating properties of heterotypic gap junction (GJ) channels 
      assembled between cells expressing wild-type Cx40, Cx43, or Cx45 and their fusion 
      forms tagged with color variants of green fluorescent protein. We show that these 
      Cxs, with exception of Cxs 40 and 43, are compatible to form functional 
      heterotypic GJ channels. Cx40 and Cx43 hemichannels are unable or effectively 
      impaired in their ability to dock and/or assemble into junctional plaques. When 
      cells expressing Cx45 contacted those expressing Cx40 or Cx43 they readily formed 
      junctional plaques with cell-cell coupling characterized by asymmetric junctional 
      conductance dependence on transjunctional voltage, V(j). Cx40/Cx45 heterotypic GJ 
      channels preferentially exhibit V(j)-dependent gating transitions between open 
      and residual states with a conductance of approximately 42 pS; transitions 
      between fully open and closed states with conductance of approximately 52 pS in 
      magnitude occur at substantially lower ( approximately 10-fold) frequency. 
      Cx40/Cx45 junctions demonstrate electrical signal transfer asymmetry that can be 
      modulated between unidirectional and bidirectional by small changes in the 
      difference between holding potentials of the coupled cells. Furthermore, both 
      fast and slow gating mechanisms of Cx40 exhibit a negative gating polarity.
FAU - Rackauskas, Mindaugas
AU  - Rackauskas M
AD  - Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, 
      USA.
FAU - Kreuzberg, Maria M
AU  - Kreuzberg MM
FAU - Pranevicius, Mindaugas
AU  - Pranevicius M
FAU - Willecke, Klaus
AU  - Willecke K
FAU - Verselis, Vytas K
AU  - Verselis VK
FAU - Bukauskas, Feliksas F
AU  - Bukauskas FF
LA  - eng
GR  - R01 MH066199/MH/NIMH NIH HHS/United States
GR  - R01 NS036706/NS/NINDS NIH HHS/United States
GR  - R01 NS036706-09/NS/NINDS NIH HHS/United States
GR  - MH66199/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20061222
PL  - United States
TA  - Biophys J
JT  - Biophysical journal
JID - 0370626
RN  - 0 (Connexin 43)
RN  - 0 (Connexins)
RN  - 0 (connexin 45)
SB  - IM
MH  - Connexin 43/chemistry/*physiology
MH  - Connexins/chemistry/*physiology
MH  - Gap Junctions/*physiology
MH  - HeLa Cells
MH  - Humans
MH  - Ion Channel Gating/*physiology
MH  - Membrane Potentials/*physiology
MH  - Structure-Activity Relationship
MH  - Gap Junction alpha-5 Protein
PMC - PMC1861779
EDAT- 2006/12/26 09:00
MHDA- 2007/05/17 09:00
PMCR- 2008/03/15
CRDT- 2006/12/26 09:00
PHST- 2006/12/26 09:00 [pubmed]
PHST- 2007/05/17 09:00 [medline]
PHST- 2006/12/26 09:00 [entrez]
PHST- 2008/03/15 00:00 [pmc-release]
AID - S0006-3495(07)71002-0 [pii]
AID - 99358 [pii]
AID - 10.1529/biophysj.106.099358 [doi]
PST - ppublish
SO  - Biophys J. 2007 Mar 15;92(6):1952-65. doi: 10.1529/biophysj.106.099358. Epub 2006 
      Dec 22.