PMID- 1718989
OWN - NLM
STAT- MEDLINE
DCOM- 19911226
LR  - 20211203
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 266
IP  - 33
DP  - 1991 Nov 25
TI  - The tyrosine-phosphorylated hepatocyte growth factor/scatter factor receptor 
      associates with phosphatidylinositol 3-kinase.
PG  - 22087-90
AB  - The receptor for hepatocyte growth factor, also known as scatter factor (HGF/SF), 
      has recently been identified as the 190-kDa heterodimeric tyrosine kinase encoded 
      by the MET proto-oncogene (p190MET). The signaling pathway(s) triggered by HGF/SF 
      are unknown. In A549 cells, a lung epithelial cell line, nanomolar concentrations 
      of HGF/SF induced tyrosine phosphorylation of the p190MET receptor. The 
      autophosphorylated receptor coprecipitated with phosphatidylinositol 3-kinase (PI 
      3-kinase) activity. In GTL16 cells, a cell line derived from a gastric carcinoma, 
      the p190MET receptor, overexpressed and constitutively phosphorylated on 
      tyrosine, coprecipitated with PI 3-kinase activity and with the 85-kDa PI 
      3-kinase subunit. In these cells activation of protein kinase C or the increase 
      of intracellular [Ca2+] inhibits tyrosine phosphorylation of the p190MET receptor 
      as well as the association with both PI 3-kinase activity and the 85-kDa subunit 
      of the enzyme. In an in vitro assay, tyrosine phosphorylation of the immobilized 
      p190MET receptor was required for binding of PI 3-kinase from cell lysates. These 
      data strongly suggest that the signaling pathway activated by the HGF/SF receptor 
      includes generation of D-3-phosphorylated inositol phospholipids.
FAU - Graziani, A
AU  - Graziani A
AD  - Department of Biomedical Sciences and Oncology, University of Torino Medical 
      School, Italy.
FAU - Gramaglia, D
AU  - Gramaglia D
FAU - Cantley, L C
AU  - Cantley LC
FAU - Comoglio, P M
AU  - Comoglio PM
LA  - eng
SI  - GENBANK/X54559
GR  - R01 GM041890/GM/NIGMS NIH HHS/United States
GR  - GM 36624/GM/NIGMS NIH HHS/United States
GR  - GM 41890/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Growth Substances)
RN  - 0 (MAS1 protein, human)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (Receptors, Cell Surface)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - 42HK56048U (Tyrosine)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.- (Phosphotransferases)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Cell Line
MH  - Growth Substances/isolation & purification/*metabolism
MH  - Hepatocyte Growth Factor
MH  - Humans
MH  - Macromolecular Substances
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Phosphatidylinositol 3-Kinases
MH  - Phosphorylation
MH  - Phosphotransferases/*metabolism
MH  - Phosphotyrosine
MH  - Protein Binding
MH  - Protein Kinase C/metabolism
MH  - Proto-Oncogene Mas
MH  - Proto-Oncogene Proteins c-met
MH  - Proto-Oncogenes
MH  - Receptors, Cell Surface/*metabolism
MH  - Tyrosine/*analogs & derivatives/analysis
EDAT- 1991/11/25 00:00
MHDA- 1991/11/25 00:01
CRDT- 1991/11/25 00:00
PHST- 1991/11/25 00:00 [pubmed]
PHST- 1991/11/25 00:01 [medline]
PHST- 1991/11/25 00:00 [entrez]
AID - S0021-9258(18)54536-1 [pii]
PST - ppublish
SO  - J Biol Chem. 1991 Nov 25;266(33):22087-90.