PMID- 17190512
OWN - NLM
STAT- MEDLINE
DCOM- 20070206
LR  - 20061227
IS  - 0743-7463 (Print)
IS  - 0743-7463 (Linking)
VI  - 23
IP  - 1
DP  - 2007 Jan 2
TI  - Characterization of superlighting polymer-DNA aggregates: a fluorescence and 
      light scattering study.
PG  - 258-64
AB  - The massive amplification of fluorescence signal observed upon hybridization of 
      as few as five DNA molecules into self-assembled structures formed between a 
      cationic polymer and DNA oligonucleotides is investigated. These superlighting 
      polymer-DNA aggregates were studied by fluorescence spectroscopy, static and 
      dynamic light scattering, and zeta potential measurements in order to 
      characterize the aggregation behavior and to understand the processes involved 
      during DNA detection. Multi-angle laser light scattering was also used to obtain 
      the weight-average aggregate mass (AM), the aggregation number (Nagg), the radius 
      of gyration (Rg), and the dissymmetry ratio (z). These results have been used, 
      together with TEM imaging, to propose a suitable physical model for the 
      aggregates.
FAU - Dore, Kim
AU  - Dore K
AD  - Department of Chemistry, Centre d'optique, photonique et laser (COPL), and Centre 
      de recherches sur la science et l'ingenierie des macromolecules (CERSIM), 
      Universite Laval, Quebec City, QC, Canada.
FAU - Neagu-Plesu, Rodica
AU  - Neagu-Plesu R
FAU - Leclerc, Mario
AU  - Leclerc M
FAU - Boudreau, Denis
AU  - Boudreau D
FAU - Ritcey, Anna M
AU  - Ritcey AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Alexa fluor 546)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Quinolinium Compounds)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/*chemistry/ultrastructure
MH  - Microscopy, Electron, Transmission
MH  - Nucleic Acid Hybridization
MH  - Oligodeoxyribonucleotides/*chemistry
MH  - Quinolinium Compounds/*chemistry
MH  - Spectrometry, Fluorescence
EDAT- 2006/12/28 09:00
MHDA- 2007/02/07 09:00
CRDT- 2006/12/28 09:00
PHST- 2006/12/28 09:00 [pubmed]
PHST- 2007/02/07 09:00 [medline]
PHST- 2006/12/28 09:00 [entrez]
AID - 10.1021/la061699a [doi]
PST - ppublish
SO  - Langmuir. 2007 Jan 2;23(1):258-64. doi: 10.1021/la061699a.