PMID- 17191078
OWN - NLM
STAT- MEDLINE
DCOM- 20070725
LR  - 20220309
IS  - 0271-678X (Print)
IS  - 0271-678X (Linking)
VI  - 27
IP  - 6
DP  - 2007 Jun
TI  - Monocyte chemoattractant protein-1 plays a critical role in neuroblast migration 
      after focal cerebral ischemia.
PG  - 1213-24
AB  - Transient focal ischemia is known to induce proliferation of neural progenitors 
      in adult rodent brain. We presently report that doublecortin positive neuroblasts 
      formed in the subventricular zone (SVZ) and the posterior peri-ventricle region 
      migrate towards the cortical and striatal penumbra after transient middle 
      cerebral artery occlusion (MCAO) in adult rodents. Cultured neural progenitor 
      cells grafted into the non-infarcted area of the ipsilateral cortex migrated 
      preferentially towards the infarct. As chemokines are known to induce cell 
      migration, we investigated if monocyte chemoattractant protein-1 (MCP-1) has a 
      role in post-ischemic neuroblast migration. Transient MCAO induced an increased 
      expression of MCP-1 mRNA in the ipsilateral cortex and striatum. Immunostaining 
      showed that the expression of MCP-1 was localized in the activated microglia and 
      astrocytes present in the ischemic areas between days 1 and 3 of reperfusion. 
      Furthermore, infusion of MCP-1 into the normal striatum induced neuroblast 
      migration to the infusion site. The migrating neuroblasts expressed the MCP-1 
      receptor CCR2. In knockout mice that lacked either MCP-1 or its receptor CCR2, 
      there was a significant decrease in the number of migrating neuroblasts from the 
      ipsilateral SVZ to the ischemic striatum. These results show that MCP-1 is one of 
      the factors that attract the migration of newly formed neuroblasts from 
      neurogenic regions to the damaged regions of brain after focal ischemia.
FAU - Yan, Yi-Ping
AU  - Yan YP
AD  - Department of Neurological Surgery, University of Wisconsin-Madison, Madison, 
      Wisconsin 53792, USA.
FAU - Sailor, Kurt A
AU  - Sailor KA
FAU - Lang, Bradley T
AU  - Lang BT
FAU - Park, Seung-Won
AU  - Park SW
FAU - Vemuganti, Raghu
AU  - Vemuganti R
FAU - Dempsey, Robert J
AU  - Dempsey RJ
LA  - eng
GR  - R01 NS044173/NS/NINDS NIH HHS/United States
GR  - R01 NS045143/NS/NINDS NIH HHS/United States
GR  - R01 NS049448/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20061227
PL  - United States
TA  - J Cereb Blood Flow Metab
JT  - Journal of cerebral blood flow and metabolism : official journal of the 
      International Society of Cerebral Blood Flow and Metabolism
JID - 8112566
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Ccr2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Dcx protein, rat)
RN  - 0 (Doublecortin Protein)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Animals
MH  - Brain Ischemia/*pathology
MH  - *Cell Movement
MH  - Cell Proliferation
MH  - Chemokine CCL2/genetics/*physiology
MH  - Doublecortin Protein
MH  - Infarction, Middle Cerebral Artery
MH  - Mice
MH  - Mice, Knockout
MH  - Neurons/cytology/*physiology
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/analysis
MH  - Up-Regulation
EDAT- 2006/12/28 09:00
MHDA- 2007/07/26 09:00
CRDT- 2006/12/28 09:00
PHST- 2006/12/28 09:00 [pubmed]
PHST- 2007/07/26 09:00 [medline]
PHST- 2006/12/28 09:00 [entrez]
AID - 9600432 [pii]
AID - 10.1038/sj.jcbfm.9600432 [doi]
PST - ppublish
SO  - J Cereb Blood Flow Metab. 2007 Jun;27(6):1213-24. doi: 10.1038/sj.jcbfm.9600432. 
      Epub 2006 Dec 27.