PMID- 17192399
OWN - NLM
STAT- MEDLINE
DCOM- 20070605
LR  - 20220129
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 109
IP  - 9
DP  - 2007 May 1
TI  - Stimulation of P2 receptors causes release of IL-1beta-loaded microvesicles from 
      human dendritic cells.
PG  - 3856-64
AB  - Dendritic cells (DCs) are professional antigen-presenting cells that initiate the 
      immune response by activating T lymphocytes. DCs express plasma membrane 
      receptors for extracellular nucleotides named P2 receptors (P2Rs). Stimulation of 
      P2Rs in these cells is known to cause chemotaxis, cytokine release, and cell 
      death and to modulate LPS-dependent differentiation. Here we show that 
      stimulation of the P2X(7) receptor subtype (P2X(7)R) causes fast microvesicle 
      shedding from DC plasma membrane. Vesicle release occurs from both immature and 
      mature DCs; however, only vesicles from mature DCs, due to their previous 
      exposure to LPS, contain IL-1beta. Microvesicles, whether from immature or mature 
      DCs, also contain caspase-1 and -3 and cathepsin D. They also express the P2X(7)R 
      in addition to other P2Rs and known markers of immune cells such as major 
      histocompatibility complex II (MHC II) and CD39. Activation of the P2X(7)R by 
      extracellular ATP causes IL-1beta release from the vesicle lumen. Previous 
      studies demonstrated that high extracellular K(+) inhibits IL-1beta processing 
      and release; here we show that high ionic strength reduces microvesicle shedding 
      when compared with a low ionic strength medium but strongly increases 
      microvesicle IL-1beta loading.
FAU - Pizzirani, Cinzia
AU  - Pizzirani C
AD  - Department of Experimental and Diagnostic Medicine, Section of General Pathology, 
      University of Ferrara, I-44100 Ferrara, Italy.
FAU - Ferrari, Davide
AU  - Ferrari D
FAU - Chiozzi, Paola
AU  - Chiozzi P
FAU - Adinolfi, Elena
AU  - Adinolfi E
FAU - Sandona, Dorianna
AU  - Sandona D
FAU - Savaglio, Erika
AU  - Savaglio E
FAU - Di Virgilio, Francesco
AU  - Di Virgilio F
LA  - eng
GR  - GGP06070/TI_/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061227
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (P2RX7 protein, human)
RN  - 0 (Purinergic P2 Receptor Agonists)
RN  - 0 (Receptors, Purinergic P2)
RN  - 0 (Receptors, Purinergic P2X7)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.36 (Caspase 1)
RN  - EC 3.4.23.5 (Cathepsin D)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Adenosine Triphosphate/*pharmacology
MH  - Caspase 1/immunology/metabolism
MH  - Caspase 3/immunology/metabolism
MH  - Cathepsin D/immunology/metabolism
MH  - Cell Differentiation/drug effects/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/immunology/*metabolism
MH  - Humans
MH  - Interleukin-1beta/immunology/*metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Potassium/immunology/metabolism
MH  - Purinergic P2 Receptor Agonists
MH  - Receptors, Purinergic P2/immunology/*metabolism
MH  - Receptors, Purinergic P2X7
MH  - Secretory Vesicles/immunology/*metabolism
EDAT- 2006/12/29 09:00
MHDA- 2007/06/06 09:00
CRDT- 2006/12/29 09:00
PHST- 2006/12/29 09:00 [pubmed]
PHST- 2007/06/06 09:00 [medline]
PHST- 2006/12/29 09:00 [entrez]
AID - S0006-4971(20)41633-7 [pii]
AID - 10.1182/blood-2005-06-031377 [doi]
PST - ppublish
SO  - Blood. 2007 May 1;109(9):3856-64. doi: 10.1182/blood-2005-06-031377. Epub 2006 
      Dec 27.