PMID- 17194151
OWN - NLM
STAT- MEDLINE
DCOM- 20070313
LR  - 20231105
IS  - 0003-2700 (Print)
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 79
IP  - 1
DP  - 2007 Jan 1
TI  - Affinity capture and detection of immunoglobulin E in human serum using an 
      aptamer-modified surface in matrix-assisted laser desorption/ionization mass 
      spectrometry.
PG  - 273-9
AB  - Capture and detection of immunoglobulin E (IgE) in simple solution and in human 
      serum using an aptamer-modified probe surface for affinity matrix-assisted laser 
      desorption/ionization mass spectroscopy detection is reported. Detectable signals 
      were obtained for 1 amol of IgE applied either in a single, 1microL application 
      of 1 pM IgE or after 10 successive, 1-microL applications of 100 fM IgE. In both 
      cases, the surface was rinsed after each application of IgE to remove sample 
      concomitants including salts and free or nonspecifically associated proteins. 
      Detection of native IgE, which is the least abundant of the serum immunoglobulins 
      and occurs at subnanomolar levels, in human serum was demonstrated and 
      interference from the high-abundance immunoglobulins and albumin was 
      investigated. The aptamer-modified surface showed high selectivity toward 
      immunoglobulins in serum, with no significant interference from serum albumin. 
      Addition of IgE to the serum suppressed the signals from the other 
      immunoglobulins, confirming the expected selectivity of the aptamer surface 
      toward IgE. Dilution of the serum increased the selectivity toward IgE; the 
      protein was detected without interference in a 10,000-fold dilution of the serum, 
      which is consistent with detection of IgE at amol (pM) levels in standard 
      solutions.
FAU - Cole, Jacquelyn R
AU  - Cole JR
AD  - Department of Chemistry and Chemical Biology, Cogswell Building, Rensselaer 
      Polytechnic Institute, Troy, New York 12180, USA.
FAU - Dick, Lawrence W Jr
AU  - Dick LW Jr
FAU - Morgan, Elizabeth J
AU  - Morgan EJ
FAU - McGown, Linda B
AU  - McGown LB
LA  - eng
GR  - R21 DK070762/DK/NIDDK NIH HHS/United States
GR  - R21 DK070762-01/DK/NIDDK NIH HHS/United States
GR  - R21 DK070762-02/DK/NIDDK NIH HHS/United States
GR  - 1 R21 DK70762-01/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Serum Albumin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Serum Albumin/analysis
MH  - Spectrometry, Mass, Matrix-Assisted Laser 
      Desorption-Ionization/*methods/standards
PMC - PMC2569142
MID - NIHMS61627
EDAT- 2006/12/30 09:00
MHDA- 2007/03/14 09:00
PMCR- 2008/10/17
CRDT- 2006/12/30 09:00
PHST- 2006/12/30 09:00 [pubmed]
PHST- 2007/03/14 09:00 [medline]
PHST- 2006/12/30 09:00 [entrez]
PHST- 2008/10/17 00:00 [pmc-release]
AID - 10.1021/ac061256b [doi]
PST - ppublish
SO  - Anal Chem. 2007 Jan 1;79(1):273-9. doi: 10.1021/ac061256b.