PMID- 17194745
OWN - NLM
STAT- MEDLINE
DCOM- 20070424
LR  - 20181201
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 148
IP  - 4
DP  - 2007 Apr
TI  - Ablation of the cholesterol transporter adenosine triphosphate-binding cassette 
      transporter G1 reduces adipose cell size and protects against diet-induced 
      obesity.
PG  - 1561-73
AB  - The ATP-binding cassette transporter G1 (ABCG1) catalyzes export of cellular 
      cholesterol from macrophages and hepatocytes. Here we identify an additional 
      function of ABCG1 in the regulation of adiposity in screens of the Drosophila 
      melanogaster and the New Zealand obese (NZO) mouse genomes. Insertion of modified 
      transposable elements of the P-family upstream of CG17646, the Drosophila 
      ortholog of Abcg1, generated lines of flies with increased triglyceride stores. 
      In NZO mice, an Abcg1 variant was identified in a suggestive adiposity 
      quantitative trait locus and was associated with higher expression of the gene in 
      white adipose tissue. Targeted disruption of Abcg1 in mice resulted in reduced 
      body weight gain (8.42+/-0.6 g in Abcg1-/- vs. 13.07+/-1.1 g in Abcg1+/+ mice) 
      and adipose tissue mass gain (3.78+/-1.3 g in Abcg1-/- vs. 9.39+/-1.6 g in 
      Abcg1+/+ mice) detected over a period of 12 wk. The reduction of adipose tissue 
      mass in Abcg1-/- mice was associated with markedly decreased size of the 
      adipocytes. In contrast to their wild-type littermates, male Abcg1-/- mice 
      exhibited no high-fat diet-induced impairment of glucose tolerance and fatty 
      liver. Furthermore, Abcg1-/- mice possess decreased food intake and elevated 
      total energy expenditure (Abcg1-/- mice, 748.1+/-5.4 kJ/kg metabolic body mass; 
      Abcg1+/+ mice, 684.3+/-5.0 kJ/kg metabolic body mass; P=0.011), body temperature 
      (Abcg1-/- mice, 37.82+/-0.29 C; Abcg1+/+ mice, 36.83+/-0.24 C; P<0.05), and 
      locomotor activity (Abcg1-/- mice, 3655+/-189 counts/12 h during dark phase; 
      Abcg1+/+ mice, 2445+/-235 counts/12 h during dark phase; P<0.01). Our data 
      indicate a previously unrecognized role of ABCG1 in the regulation of energy 
      balance and triglyceride storage.
FAU - Buchmann, Jana
AU  - Buchmann J
AD  - Department of Pharmacology, German Institute of Human Nutrition, 
      Potsdam-Rehbruecke, Arthur-Scheunert-Allee 114-116, D-14558 Nuthetal, Germany.
FAU - Meyer, Christoph
AU  - Meyer C
FAU - Neschen, Susanne
AU  - Neschen S
FAU - Augustin, Robert
AU  - Augustin R
FAU - Schmolz, Katja
AU  - Schmolz K
FAU - Kluge, Reinhart
AU  - Kluge R
FAU - Al-Hasani, Hadi
AU  - Al-Hasani H
FAU - Jurgens, Hella
AU  - Jurgens H
FAU - Eulenberg, Karsten
AU  - Eulenberg K
FAU - Wehr, Roland
AU  - Wehr R
FAU - Dohrmann, Cord
AU  - Dohrmann C
FAU - Joost, Hans-Georg
AU  - Joost HG
FAU - Schurmann, Annette
AU  - Schurmann A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061228
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (ABCG1 protein, mouse)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Lipoproteins)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1
MH  - ATP-Binding Cassette Transporters/*genetics/physiology
MH  - Adipocytes/*cytology
MH  - Adipose Tissue/metabolism
MH  - Animals
MH  - Body Weight
MH  - *Cell Size
MH  - Diet/*adverse effects
MH  - Drosophila melanogaster
MH  - Female
MH  - Lipoproteins/*genetics/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred NZB
MH  - Mice, Knockout
MH  - Mice, Obese
MH  - Obesity/etiology/genetics/*prevention & control
EDAT- 2006/12/30 09:00
MHDA- 2007/04/25 09:00
CRDT- 2006/12/30 09:00
PHST- 2006/12/30 09:00 [pubmed]
PHST- 2007/04/25 09:00 [medline]
PHST- 2006/12/30 09:00 [entrez]
AID - en.2006-1244 [pii]
AID - 10.1210/en.2006-1244 [doi]
PST - ppublish
SO  - Endocrinology. 2007 Apr;148(4):1561-73. doi: 10.1210/en.2006-1244. Epub 2006 Dec 
      28.