PMID- 17194774
OWN - NLM
STAT- MEDLINE
DCOM- 20071015
LR  - 20181113
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 175
IP  - 3
DP  - 2007 Mar
TI  - Evolution of different Y chromosomes in two medaka species, Oryzias dancena and 
      O. latipes.
PG  - 1335-40
AB  - Although the sex-determining gene DMY has been identified on the Y chromosome in 
      the medaka (Oryzias latipes), this gene is absent in most Oryzias species, 
      suggesting that closely related species have different sex-determining genes. 
      Here, we investigated the sex-determination mechanism in O. dancena, which does 
      not possess the DMY gene. Since heteromorphic sex chromosomes have not been 
      reported in this species, a progeny test of sex-reversed individuals produced by 
      hormone treatment was performed. Sex-reversed males yielded all-female progeny, 
      indicating that O. dancena has an XX/XY sex-determination system. To uncover the 
      cryptic sex chromosomes, sex-linked DNA markers were screened using expressed 
      sequence tags (ESTs) established in O. latipes. Linkage analysis of isolated 
      sex-linked ESTs showed a conserved synteny between the sex chromosomes in O. 
      dancena and an autosome in O. latipes. Fluorescence in situ hybridization (FISH) 
      analysis of these markers confirmed that sex chromosomes of these species are not 
      homologous. These findings strongly suggest an independent origin of sex 
      chromosomes in O. dancena and O. latipes. Further analysis of the sex-determining 
      region in O. dancena should provide crucial insights into the evolution of 
      sex-determination mechanisms in vertebrates.
FAU - Takehana, Yusuke
AU  - Takehana Y
AD  - Graduate School of Science and Technology, Niigata University, Ikarashi, Niigata 
      950-2181, Japan. yusuke@env.sc.niigata-u.ac.jp
FAU - Demiyah, Diana
AU  - Demiyah D
FAU - Naruse, Kiyoshi
AU  - Naruse K
FAU - Hamaguchi, Satoshi
AU  - Hamaguchi S
FAU - Sakaizumi, Mitsuru
AU  - Sakaizumi M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061228
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - Crosses, Genetic
MH  - *Evolution, Molecular
MH  - Expressed Sequence Tags
MH  - Female
MH  - Hermaphroditic Organisms
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Oryzias/*genetics
MH  - *Sex Determination Processes
MH  - Species Specificity
MH  - Synteny/genetics
MH  - Y Chromosome/*genetics
PMC - PMC1840079
EDAT- 2006/12/30 09:00
MHDA- 2007/10/16 09:00
PMCR- 2007/06/01
CRDT- 2006/12/30 09:00
PHST- 2006/12/30 09:00 [pubmed]
PHST- 2007/10/16 09:00 [medline]
PHST- 2006/12/30 09:00 [entrez]
PHST- 2007/06/01 00:00 [pmc-release]
AID - genetics.106.068247 [pii]
AID - gen17531335 [pii]
AID - 10.1534/genetics.106.068247 [doi]
PST - ppublish
SO  - Genetics. 2007 Mar;175(3):1335-40. doi: 10.1534/genetics.106.068247. Epub 2006 
      Dec 28.