PMID- 17197172
OWN - NLM
STAT- MEDLINE
DCOM- 20070511
LR  - 20220309
IS  - 0960-0760 (Print)
IS  - 0960-0760 (Linking)
VI  - 103
IP  - 3-5
DP  - 2007 Mar
TI  - Activation of MAPKs by 1alpha,25(OH)2-Vitamin D3 and 17beta-estradiol in skeletal 
      muscle cells leads to phosphorylation of Elk-1 and CREB transcription factors.
PG  - 462-6
AB  - The mitogen activated protein kinases (MAPKs) have been classified into at least 
      six subfamilies, among which ERK1/2, JNK1/2 and p38 MAPK are the most extensively 
      studied. The steroid hormones 1alpha,25-dihydroxy-Vitamin D(3) and 
      17beta-estradiol promote biological responses through activation of MAPK cascades 
      in various cell types. We previously reported that 1alpha,25(OH)(2)D(3) rapidly 
      (within 1 min) activates p38 MAPK in C2C12 skeletal muscle cells. In this work, 
      using the same muscle cell line, we demonstrate that 1alpha,25(OH)(2)D(3) or 
      17beta-estradiol phosphorylate and activate ERK1/2 and p38 MAPK after longer 
      treatment intervals, maximal effects seen at 90 and 30 min (ERK1/2) and at 60 and 
      15 min (p38 MAPK) for these hormones, respectively. Hormone-dependent ERK and p38 
      activation was abolished by MAPK specific inhibitors U0126 and SB203580. 
      1alpha,25(OH)(2)D(3) and 17beta-estradiol also induced the phosphorylation of 
      CREB and Elk-1 transcription factors in an ERK1/2-dependent manner. Simultaneous 
      addition of both hormones potentiated CREB phosphorylation. 1alpha,25(OH)(2)D(3)- 
      and 17beta-estradiol-induced c-fos expression, which was mediated by p38 
      phosphorylation. The action of 17beta-estradiol on c-fos levels was also 
      dependent on ERK1/2. These results suggest that MAPK signalling pathways play an 
      important role in regulating early gene expression through CREB and Elk-1 
      activation in skeletal muscle cells.
FAU - Ronda, Ana C
AU  - Ronda AC
AD  - Departamento de Biologia, Bioquimica y Farmacia, Universidad Nacional del Sur. 
      8000 Bahia Blanca, Argentina.
FAU - Buitrago, Claudia
AU  - Buitrago C
FAU - Colicheo, Andrea
AU  - Colicheo A
FAU - de Boland, Ana R
AU  - de Boland AR
FAU - Roldan, Emilio
AU  - Roldan E
FAU - Boland, Ricardo
AU  - Boland R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070102
PL  - England
TA  - J Steroid Biochem Mol Biol
JT  - The Journal of steroid biochemistry and molecular biology
JID - 9015483
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (ets-Domain Protein Elk-1)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Animals
MH  - Calcitriol/*pharmacology
MH  - Cell Line
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - Enzyme Activation/drug effects
MH  - Estradiol/*pharmacology
MH  - Mice
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Muscle, Skeletal/*drug effects/*metabolism
MH  - Phosphorylation/drug effects
MH  - Proto-Oncogene Proteins c-fos/metabolism
MH  - Signal Transduction
MH  - ets-Domain Protein Elk-1/*metabolism
EDAT- 2007/01/02 09:00
MHDA- 2007/05/12 09:00
CRDT- 2007/01/02 09:00
PHST- 2007/01/02 09:00 [pubmed]
PHST- 2007/05/12 09:00 [medline]
PHST- 2007/01/02 09:00 [entrez]
AID - S0960-0760(06)00362-1 [pii]
AID - 10.1016/j.jsbmb.2006.11.005 [doi]
PST - ppublish
SO  - J Steroid Biochem Mol Biol. 2007 Mar;103(3-5):462-6. doi: 
      10.1016/j.jsbmb.2006.11.005. Epub 2007 Jan 2.